About: Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A large epidemic caused by shigatoxigenic Escherichia coli (E. coli) in spring 2011 in Germany resulted in reduction of trust in the health safety of raw vegetables and sprouted seeds. This study focused on the detection and characterization of E. coli in raw vegetables and sprouted seeds sold in the Czech Republic. Out of 91 samples, 24 (26.4%) were positive for the presence of E. coli. Resistance to antimicrobial agents was determined by the disk diffusion method and E-test. Polymerase chain reaction was used for the detection of selected genes encoding virulence - eaeA, hly, stx(1), and stx(2) and genes encoding resistance to tetracycline - tet(A), tet(B), tet(C), and tet(G) and to beta-lactams - bla(TEM), bla(SHV), and bla(CTX). The bla(TEM) gene was detected in two isolates, the tet(B) gene in three and tet(A) in one isolate. No hly, stx(1), or stx(2) genes were present, but the eaeA gene was found in three (11.1%) isolates from imported vegetables. These isolates can be considered as potentially enteropathogenic. Results of this study show that raw vegetables and sprouted seeds sold in the retail market can represent a potential risk for consumers. A large epidemic caused by shigatoxigenic Escherichia coli (E. coli) in spring 2011 in Germany resulted in reduction of trust in the health safety of raw vegetables and sprouted seeds. This study focused on the detection and characterization of E. coli in raw vegetables and sprouted seeds sold in the Czech Republic. Out of 91 samples, 24 (26.4%) were positive for the presence of E. coli. Resistance to antimicrobial agents was determined by the disk diffusion method and E-test. Polymerase chain reaction was used for the detection of selected genes encoding virulence - eaeA, hly, stx(1), and stx(2) and genes encoding resistance to tetracycline - tet(A), tet(B), tet(C), and tet(G) and to beta-lactams - bla(TEM), bla(SHV), and bla(CTX). The bla(TEM) gene was detected in two isolates, the tet(B) gene in three and tet(A) in one isolate. No hly, stx(1), or stx(2) genes were present, but the eaeA gene was found in three (11.1%) isolates from imported vegetables. These isolates can be considered as potentially enteropathogenic. Results of this study show that raw vegetables and sprouted seeds sold in the retail market can represent a potential risk for consumers. (en)
Title	Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic (en)
skos:prefLabel	Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic Characteristics of Escherichia coli from raw vegetables at a retail market in the Czech Republic (en)
skos:notation	RIV/00027162:_____/13:#0001003!RIV14-MZE-00027162
http://linked.open...avai/predkladatel	Výzkumný ústav veterinárního lékařství, v.v.i,
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), V, Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Karpíšková, Renáta Koláčková, Ivana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	65031
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/13:#0001003
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Vegetables; Virulence genes; Antibiotic resistance (en)
http://linked.open.../riv/klicoveSlovo	Virulence genes Vegetables Antibiotic resistance
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[97A125C8C46D]
http://linked.open...i/riv/nazevZdroje	International Journal of Food Microbiology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	167
http://linked.open...iv/tvurceVysledku	Karpíšková, Renáta Koláčková, Ivana Cupáková, Š. Skočková, A.
http://linked.open...ain/vavai/riv/wos	000327109500011
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0168-1605
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.ijfoodmicro.2013.09.011

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