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  • Numerous foodborne outbreaks due to consumption of berry fruit contaminated by human enteric viruses have been reported. This European multinational study investigated possible contamination routes by monitoring the entire food chain for a panel of human and animal enteric viruses. A total of 785 samples were collected throughout the food production chain of the Czech Republic, Finland, Poland and Serbia during two growing seasons. Samples collected during the production phase, the processing phase, and at point-of-sale included irrigation water, animal faeces, raspberries or strawberries, and swabs from food handlers' hand, toilets and conveyor belts. Presence of human (adenoviruses, hAdV norovirus, NoV GI, NoV GII and hepatitis A virus, HAV), animal (porcine adenovirus, pAdV and bovine polyomavirus, bPyV) and zoonotic (hepatitis E virus, HEV) viruses was tested by real time (reverse transcription) PCR. hAdV was found in 9.5%, 5.8% and 9.1% of samples of irrigation water, food handlers' hands and toilets, respectively. The prevalence of hAdV in fresh raspberries, frozen raspberries and fresh strawberries, was 0.7%, 3.2% and 2.0%, respectively. NoV GII was detected in two (3.6%) water samples. HAV was not detected in any of analysed samples. HEV-contaminated frozen raspberries were found once (2.6%). Animal faecal contamination was evidenced by positive pAdV and bPyV assay results; one water sample contained both viruses, and 5.7% and 13% of fresh and frozen berries were positive for pAdV. hAdV was found both in irrigation water and on food handler's hands, which indicated that these may be important vehicles by which human pathogenic viruses enter the berry fruit chain. This study gives insight into viral sources and transmission routes and emphasizes the necessity for thorough compliance with good agricultural and hygienic practice at the farms to help protect the public from viral infections.
  • Numerous foodborne outbreaks due to consumption of berry fruit contaminated by human enteric viruses have been reported. This European multinational study investigated possible contamination routes by monitoring the entire food chain for a panel of human and animal enteric viruses. A total of 785 samples were collected throughout the food production chain of the Czech Republic, Finland, Poland and Serbia during two growing seasons. Samples collected during the production phase, the processing phase, and at point-of-sale included irrigation water, animal faeces, raspberries or strawberries, and swabs from food handlers' hand, toilets and conveyor belts. Presence of human (adenoviruses, hAdV norovirus, NoV GI, NoV GII and hepatitis A virus, HAV), animal (porcine adenovirus, pAdV and bovine polyomavirus, bPyV) and zoonotic (hepatitis E virus, HEV) viruses was tested by real time (reverse transcription) PCR. hAdV was found in 9.5%, 5.8% and 9.1% of samples of irrigation water, food handlers' hands and toilets, respectively. The prevalence of hAdV in fresh raspberries, frozen raspberries and fresh strawberries, was 0.7%, 3.2% and 2.0%, respectively. NoV GII was detected in two (3.6%) water samples. HAV was not detected in any of analysed samples. HEV-contaminated frozen raspberries were found once (2.6%). Animal faecal contamination was evidenced by positive pAdV and bPyV assay results; one water sample contained both viruses, and 5.7% and 13% of fresh and frozen berries were positive for pAdV. hAdV was found both in irrigation water and on food handler's hands, which indicated that these may be important vehicles by which human pathogenic viruses enter the berry fruit chain. This study gives insight into viral sources and transmission routes and emphasizes the necessity for thorough compliance with good agricultural and hygienic practice at the farms to help protect the public from viral infections. (en)
Title
  • Tracing enteric viruses in the European berry fruit supply chain
  • Tracing enteric viruses in the European berry fruit supply chain (en)
skos:prefLabel
  • Tracing enteric viruses in the European berry fruit supply chain
  • Tracing enteric viruses in the European berry fruit supply chain (en)
skos:notation
  • RIV/00027162:_____/13:#0000977!RIV14-MSM-00027162
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • P(ED0006/01/01)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...dnocenehoVysledku
  • 111455
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/13:#0000977
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Foodborne viruses; Berry fruit; Food monitoring; Real-time PCR; hAdV; NoV (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [85BBD4812A72]
http://linked.open...i/riv/nazevZdroje
  • International Journal of Food Microbiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...v/svazekPeriodika
  • 167
http://linked.open...iv/tvurceVysledku
  • Pavlík, Ivo
  • Vašíčková, Petra
  • Cook, N.
  • Bouwknegt, M.
  • Kaupke, A.
  • Kozyra, I.
  • Lazic, S.
  • Maunula, L.
  • Moloney, R.
  • Petrovic, T.
  • Rutjes, S.
  • Soderberg, K.
http://linked.open...ain/vavai/riv/wos
  • 000327109500009
issn
  • 0168-1605
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.ijfoodmicro.2013.09.003
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