About: Male-specific repeats in wild Bovidae

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies. In this study, we investigated repetitive sequences localized on Y chromosomes. Repetitive DNA sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. Efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for Y chromosome separation, followed by polymerase chain reaction (PCR), cloning, and sequence analysis. We applied the derived repeat units to members of nine tribes within the Bovidae. Apart from the Y chromosomes of Bos taurus and Bubalus bubalis, where we used known sequences of repetition, the derived sequences were used as probes for fluorescent in situ cross-hybridization to members of the nine tribes of the Bovidae. We investigated the distribution of repeat units within the tribes and their localization on the Y chromosome. Sharing of sequence variants would indicate common descent, while the rapid horizontal evolution should allow discrimination between closely related species or subspecies. (en)
Title	Male-specific repeats in wild Bovidae Male-specific repeats in wild Bovidae (en)
skos:prefLabel	Male-specific repeats in wild Bovidae Male-specific repeats in wild Bovidae (en)
skos:notation	RIV/00027162:_____/12:#0000950!RIV13-MZE-00027162
http://linked.open...avai/predkladatel	Výzkumný ústav veterinárního lékařství, v.v.i,
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GAP506/10/0421), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Černohorská, Halina Rubeš, Jiří Kubíčková, Svatava Čábelová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	147911
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/12:#0000950
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Y-CHROMOSOME; CENTROMERIC REPEATS; FARM-ANIMALS; SATELLITE-I; DNA; EVOLUTION; BOVINE; SEQUENCE; SHEEP; ANEUPLOIDY (en)
http://linked.open.../riv/klicoveSlovo	SEQUENCE DNA Y-CHROMOSOME SHEEP EVOLUTION ANEUPLOIDY BOVINE CENTROMERIC REPEATS FARM-ANIMALS SATELLITE-I
http://linked.open...odStatuVydavatele	GE - Gruzie
http://linked.open...ontrolniKodProRIV	[A149932D1428]
http://linked.open...i/riv/nazevZdroje	Journal of Applied Genetics
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Phylogenetic relationships in the family Bovidae studied by analysis of subfamily specific DNA repeats and karyotype
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Kubíčková, Svatava Rubeš, Jiří Černohorská, Halina Čábelová, Kateřina
http://linked.open...ain/vavai/riv/wos	000310227900005
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	1234-1983
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s13353-012-0108-y
is http://linked.open...avai/riv/vysledek of	Male-specific repeats in wild Bovidae

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