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  • Sperm plasma membrane integrity is commonly evaluated by intravital staining tests or by a test of membrane tolerance and resistance to hypo-osmotic conditions (HOS test). We proved that despite good sperm viability as detected by a simple HOS test, the degree of membrane resistance can be low. The difference in sperm survival rates during the 120-min test between groups of boar ejaculates containing higher and lower percentages of HOS-positive and eosin-negative sperm was statistically significant even though the other parameters of sperm analysis did not significantly differ. Moreover a higher percentage of sperm was recovered after thawing of bull ejaculates with a higher percentage of HOS-positive and eosin-negative sperm. The HOS test in combination with intravital staining is a procedure providing additional information concerning dynamic changes in the membrane resistance. It allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation.
  • Sperm plasma membrane integrity is commonly evaluated by intravital staining tests or by a test of membrane tolerance and resistance to hypo-osmotic conditions (HOS test). We proved that despite good sperm viability as detected by a simple HOS test, the degree of membrane resistance can be low. The difference in sperm survival rates during the 120-min test between groups of boar ejaculates containing higher and lower percentages of HOS-positive and eosin-negative sperm was statistically significant even though the other parameters of sperm analysis did not significantly differ. Moreover a higher percentage of sperm was recovered after thawing of bull ejaculates with a higher percentage of HOS-positive and eosin-negative sperm. The HOS test in combination with intravital staining is a procedure providing additional information concerning dynamic changes in the membrane resistance. It allows assessment of semen quality from the perspective of its further use, e.g. cryopreservation. (en)
Title
  • Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test
  • Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test (en)
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  • Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test
  • Evaluation of sperm membrane resistance in boars and bulls by the modified HOS test (en)
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  • RIV/00027162:_____/11:#0000847!RIV12-MZE-00027162
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  • 198362
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  • RIV/00027162:_____/11:#0000847
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  • sperm; HOS test; membrane resistance; bull; boar (en)
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  • [661C4C27AD74]
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  • Přinosilová, Petra
  • Hlavicová, Jaroslava
  • Kopecká, Věra
  • Věžník, Zdeněk
  • Zajícová, Atanáska
http://linked.open...n/vavai/riv/zamer
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