About: Elimination of mycoplasma contamination of virus stocks

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We studied the effectivity of a commercial antibiotic kit Mycokill AB for the elimination of mycoplasma contamination in virus strains. The contaminated virus strains were first filtered, treated with Mycokill AB for three hours and repeatedly passaged in its presence in the cultivation medium in pure cell lines. Three passages in the presence of Mycokill AB were invariably followed by three passages without Mycokill AB. The effectivity of purification was then checked by PCR. Twenty-four out of 28 tested virus strains became free of mycoplasma after a first or second cycle of the treatment with Mycokill AB. The other four strains remained positive even after repeated passages. In these cases of a likely resistance to Mycokill AB, we managed to eliminate the mycoplasma contamination through a subsequent treatment with the antibiotic combination BM-Cyclin. Mycokill AB was shown in the elimination of mycoplasma from virus suspensions as successful as other known most effective antibiotics. We studied the effectivity of a commercial antibiotic kit Mycokill AB for the elimination of mycoplasma contamination in virus strains. The contaminated virus strains were first filtered, treated with Mycokill AB for three hours and repeatedly passaged in its presence in the cultivation medium in pure cell lines. Three passages in the presence of Mycokill AB were invariably followed by three passages without Mycokill AB. The effectivity of purification was then checked by PCR. Twenty-four out of 28 tested virus strains became free of mycoplasma after a first or second cycle of the treatment with Mycokill AB. The other four strains remained positive even after repeated passages. In these cases of a likely resistance to Mycokill AB, we managed to eliminate the mycoplasma contamination through a subsequent treatment with the antibiotic combination BM-Cyclin. Mycokill AB was shown in the elimination of mycoplasma from virus suspensions as successful as other known most effective antibiotics. (en)
Title	Elimination of mycoplasma contamination of virus stocks Elimination of mycoplasma contamination of virus stocks (en)
skos:prefLabel	Elimination of mycoplasma contamination of virus stocks Elimination of mycoplasma contamination of virus stocks (en)
skos:notation	RIV/00027162:_____/11:#0000774!RIV12-MZE-00027162
http://linked.open...avai/predkladatel	Výzkumný ústav veterinárního lékařství, v.v.i,
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0006/01/01), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Reichelová, Markéta Dvořáková, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	197268
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/11:#0000774
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Mycokill AB; BM-Cyclin; sparfloxacin; virus stock contamination (en)
http://linked.open.../riv/klicoveSlovo	BM-Cyclin Mycokill AB sparfloxacin virus stock contamination
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[AE2C9082E3F7]
http://linked.open...i/riv/nazevZdroje	Veterinární Medicína
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Center for Advanced Microbiology & Immunology Research in Veterinary Medicine
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	56
http://linked.open...iv/tvurceVysledku	Malenovská, Hana Reichelová, Markéta
http://linked.open...ain/vavai/riv/wos	000298506800003
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0375-8427
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Elimination of mycoplasma contamination of virus stocks Elimination of mycoplasma contamination of virus stocks

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