About: Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study, we develop the HPLC assay for the direct determination of alpha-tocopheryl succinate (TOS) entrapped in the liposomes as well as monitoring of TOS chemical stability in the liposomal lyophilisates during 6-month storage period. The composition of mobile phase was optimised for separation of TOS, alpha-tocopherol (TOH) as TOS degradation product and alpha-tocopheryl acetate as internal standard. The quantification of TOS in liposomal formulation and liposomal sediment enables to calculate entrappment efficiency of TOS (EETOS). For the liposomal lyophilisates stored during 6 months, the obtained values of EETOS were higher than 90%. The chemical stability of liposomal entrapped TOS was investigated by monitoring the level of TOH. Generally, the content of liposomal TOH did not exceed 5% of the liposomal TOS content. In this study, we develop the HPLC assay for the direct determination of alpha-tocopheryl succinate (TOS) entrapped in the liposomes as well as monitoring of TOS chemical stability in the liposomal lyophilisates during 6-month storage period. The composition of mobile phase was optimised for separation of TOS, alpha-tocopherol (TOH) as TOS degradation product and alpha-tocopheryl acetate as internal standard. The quantification of TOS in liposomal formulation and liposomal sediment enables to calculate entrappment efficiency of TOS (EETOS). For the liposomal lyophilisates stored during 6 months, the obtained values of EETOS were higher than 90%. The chemical stability of liposomal entrapped TOS was investigated by monitoring the level of TOH. Generally, the content of liposomal TOH did not exceed 5% of the liposomal TOS content. (en)
Title	Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate (en)
skos:prefLabel	Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate (en)
skos:notation	RIV/00027162:_____/10:#0000657!RIV11-AV0-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KAN200520703), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	Supll. 16
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Turánek, Jaroslav Koudelka, Štěpán Mašek, Josef Turánek-Knötigová, Pavlína
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	254540
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000657
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	alpha-tocopheryl succinate; liposomes; stability; lyophilisation; high-performance liquid chromatography (en)
http://linked.open.../riv/klicoveSlovo	stability high-performance liquid chromatography liposomes alpha-tocopheryl succinate lyophilisation
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[6A8B848E3564]
http://linked.open...i/riv/nazevZdroje	Chemické listy
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The use of ultrasound in nanomedicine
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	104
http://linked.open...iv/tvurceVysledku	Koudelka, Štěpán Mašek, Josef Turánek, Jaroslav Turánek-Knötigová, Pavlína Neužil, J.
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0009-2770
number of pages	3 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Direct high-performance liquid chromatography analysis of lyophilised liposomal entrapped alpha-tocopheryl succinate

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