About: Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction (AR) development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1 mM and 2 mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability, chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. It can be concluded that there is a potential relationship between the acrosome reaction progress in frozen-thawed boar spermatozoa and the efficiency of fertilization of porcine oocytes. A faster AR induced in spermatozoa by 1 mM caffeine resulted in a higher monospermy rate and total efficiency of fertilization. It is important to test sires before their semen is used for in vitro embryo production. The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction (AR) development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1 mM and 2 mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability, chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. It can be concluded that there is a potential relationship between the acrosome reaction progress in frozen-thawed boar spermatozoa and the efficiency of fertilization of porcine oocytes. A faster AR induced in spermatozoa by 1 mM caffeine resulted in a higher monospermy rate and total efficiency of fertilization. It is important to test sires before their semen is used for in vitro embryo production. (en)
Title	Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization (en)
skos:prefLabel	Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization (en)
skos:notation	RIV/00027162:_____/10:#0000619!RIV11-GA0-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD523/08/H064), P(QI101A166), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Rečková, Zuzana Machatková, Marie Ješeta, Michal Hulínská, Pavlína Martečíková, Soňa
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	255865
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000619
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cryopreserved spermatozoa; AR progress; porcine IVF (en)
http://linked.open.../riv/klicoveSlovo	AR progress cryopreserved spermatozoa porcine IVF
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[89FDED42E5BA]
http://linked.open...i/riv/nazevZdroje	Veterinární Medicína
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Biotechnology in pig husbandry and breeding Biotechnologies of mammalian gametes
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Hulínská, Pavlína Ješeta, Michal Machatková, Marie Martečíková, Soňa Rečková, Zuzana Pavlík, A.
http://linked.open...ain/vavai/riv/wos	000282708500003
http://linked.open...n/vavai/riv/zamer	Animal disease research, prevention and food chain protection
issn	0375-8427
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization

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