About: Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A rabbit polyclonal antibody (PAb) raised against European catfish virus (ECV; isolated from black bullhead Ameiurus melas in France) was produced and then evaluated using a panel of 9 ranavirus isolates collected from different lower vertebrate species. Using ranavirus-infected epithelioma papillosum cyprini (EPC) cell cultures, the specificity of the PAb was determined by western blot, immunogold electron microscopy, and direct enzyme-linked immunosorbent assay (ELISA). The PAb detected all tested ranaviruses except for 2 Santee-Cooper ranaviruses. The direct ELISA enabled the detection of ranavirus from a concentration of 103.5 to 103.8 TCID50 ml-1 cell culture. The results of this study revealed that the rabbit PAb against ECV could be useful for the development of specific and standardized diagnostic assays for the detection of ranaviruses from freshwater fish and amphibians. A rabbit polyclonal antibody (PAb) raised against European catfish virus (ECV; isolated from black bullhead Ameiurus melas in France) was produced and then evaluated using a panel of 9 ranavirus isolates collected from different lower vertebrate species. Using ranavirus-infected epithelioma papillosum cyprini (EPC) cell cultures, the specificity of the PAb was determined by western blot, immunogold electron microscopy, and direct enzyme-linked immunosorbent assay (ELISA). The PAb detected all tested ranaviruses except for 2 Santee-Cooper ranaviruses. The direct ELISA enabled the detection of ranavirus from a concentration of 103.5 to 103.8 TCID50 ml-1 cell culture. The results of this study revealed that the rabbit PAb against ECV could be useful for the development of specific and standardized diagnostic assays for the detection of ranaviruses from freshwater fish and amphibians. (en)
Title	Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians (en)
skos:prefLabel	Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians (en)
skos:notation	RIV/00027162:_____/10:#0000614!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Reschová, Stanislava Veselý, Tomáš Matějíčková-Cinková, Kateřina Kulich, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	257790
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000614
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Iridoviridae; Ranavirus; Polyclonal antibody; ELISA; Freshwater fish; Ornamental fish; Amphibians (en)
http://linked.open.../riv/klicoveSlovo	Ranavirus ELISA Freshwater fish Iridoviridae Ornamental fish Polyclonal antibody Amphibians
http://linked.open...odStatuVydavatele	GE - Gruzie
http://linked.open...ontrolniKodProRIV	[D1B061446A93]
http://linked.open...i/riv/nazevZdroje	Diseases of Aquatic Organisms
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	89
http://linked.open...iv/tvurceVysledku	Reschová, Stanislava Veselý, Tomáš Kulich, Pavel Cinková, Kateřina
http://linked.open...ain/vavai/riv/wos	000277332700001
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0177-5103
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians Evaluation of a polyclonal antibody for the detection and identification of ranaviruses from freshwater fish and amphibians

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software