About: In-house validation of an ELISA method for screening of semicarbazide in eggs

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Developed enzyme-linked immunosorbent assay (ELISA) method is described for the semi-quantitative determination of semicarbazide (SEM), the marker residue for the banned nitrofuran drug, nitrofurazone, in chicken eggs. Detection capability (CCbeta) determinated according to Commission Decision 2002/657/EC was determined to be 0.3 mg/kg.. A good correlation between the results obtained from ELISA and LC-MS/MS was observed in samples collected from chickens fed with a medicated ration of nitrofurazone.Validated ELISA enabled reliable monitoring of SEM levels in eggs collected from incurred chickens over a 90-day period. Developed enzyme-linked immunosorbent assay (ELISA) method is described for the semi-quantitative determination of semicarbazide (SEM), the marker residue for the banned nitrofuran drug, nitrofurazone, in chicken eggs. Detection capability (CCbeta) determinated according to Commission Decision 2002/657/EC was determined to be 0.3 mg/kg.. A good correlation between the results obtained from ELISA and LC-MS/MS was observed in samples collected from chickens fed with a medicated ration of nitrofurazone.Validated ELISA enabled reliable monitoring of SEM levels in eggs collected from incurred chickens over a 90-day period. (en) Je popsána vyvinutá ELISA (Enzyme-Linked Immunosorbent Assay) metoda pro semikvantitativni stanovení semikarbazidu (SEM), který je residuálním markerem zakázaného nitrofuranového léčiva nitrofurazonu ve vejcích. Detekční schopnost (CCbeta) validované ELISA stanovená podle Commission Decision 2002/657/EC byla 0.3 mg/kg. Velmi dobrá korelace mezi výsledky získanými pomocí ELISA a LC-MS/MS metody byla nalezena u vzorků vajec nosnic, které byly krmeny medikovanými dávkami nitrofurazonu. Validovaná ELISA umožnila spolehlivý monitoring úrovní semikarbazidu ve vejcích, které byly odebrány od uměle kontaminovaných slepic v průběhu 90 dní. (cs)
Title	In-house validation of an ELISA method for screening of semicarbazide in eggs In-house validation of an ELISA method for screening of semicarbazide in eggs (en) In-house validace ELISA metody pro screening semikarbazidu ve vejcích (cs)
skos:prefLabel	In-house validation of an ELISA method for screening of semicarbazide in eggs In-house validation of an ELISA method for screening of semicarbazide in eggs (en) In-house validace ELISA metody pro screening semikarbazidu ve vejcích (cs)
skos:notation	RIV/00027162:_____/08:#0000443!RIV09-MZE-00027162
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Diblíková, Iva Vass, Maria Hruška, Karel Fránek, Milan Kok, Eloise H.
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	372481
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/08:#0000443
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	nitrofurazone; semicarbazide; egg homogenate; ELISA; LC-MS/MS; validation; incurred samples; SEM depletion (en)
http://linked.open.../riv/klicoveSlovo	LC-MS/MS ELISA validation nitrofurazone SEM depletion egg homogenate incurred samples semicarbazide
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[2C4B0FB2595B]
http://linked.open...i/riv/nazevZdroje	Food Additives and Contaminants
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	25
http://linked.open...iv/tvurceVysledku	Hruška, Karel Diblíková, Iva Fránek, Milan Šťastný, K. Kok, Eloise H. Vass, Maria Frgalová, K.
http://linked.open...ain/vavai/riv/wos	000257741300002
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0265-203X
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	In-house validation of an ELISA method for screening of semicarbazide in eggs In-house validation of an ELISA method for screening of semicarbazide in eggs In-house validation of an ELISA method for screening of semicarbazide in eggs In-house validation of an ELISA method for screening of semicarbazide in eggs

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