About: Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting     Goto   Sponge   NotDistinct   Permalink

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  • An AI Ayrshire bull was subjected to cytogenetic examination due to lowered fertility. Preliminary Giemsa staining revealed a normal chromosome complement (60,XY) and G-banding did not allow us to draw a clear conclusion concerning an occurrence of chromosome rearrangement. Testicles were collected at slaughter and synaptonemal complex (SC) analysis revealed a large cross-shaped tetravalent configuration in pachytene spreads. No association between the tetravalent and XY bivalent was observed. Chromosome painting, with the use of bovine whole chromosome painting probes, conjugated with DAPI staining, facilitated a detailed description of the translocation rcp(2;4)(q45;q34). This study shows that post mortem analysis of synaptonemal complexes is a simple and useful tool for the preliminary detection of reciprocal translocation carriers.
  • An AI Ayrshire bull was subjected to cytogenetic examination due to lowered fertility. Preliminary Giemsa staining revealed a normal chromosome complement (60,XY) and G-banding did not allow us to draw a clear conclusion concerning an occurrence of chromosome rearrangement. Testicles were collected at slaughter and synaptonemal complex (SC) analysis revealed a large cross-shaped tetravalent configuration in pachytene spreads. No association between the tetravalent and XY bivalent was observed. Chromosome painting, with the use of bovine whole chromosome painting probes, conjugated with DAPI staining, facilitated a detailed description of the translocation rcp(2;4)(q45;q34). This study shows that post mortem analysis of synaptonemal complexes is a simple and useful tool for the preliminary detection of reciprocal translocation carriers. (en)
  • U býka Ayrshirského plemene bylo provedeno cytogenetické vyšetření z důvodu jeho snížené fertility. Po barvení Giemsou byl zjištěný normální karyotyp (60,XY) and G-pruhování neodhalilo žádnou chromozomální přestavbu. Po porážce byla odebrána varlata, analýza synaptonemálních komplexů odhalila velký tetravalent v pachytenních buňkách. Žádné spojení mezi tetravalentem a XY bivalentem nebylo pozorováno. Hybridizace s chromozom-specifickými paintingovými sondami umožnila detailní popis translokace rcp(2;4)(q45;q34). Studie ukázala, že post mortem analýza synaptonemálního kompexu je jednoduchá a užitečná metoda pro předběžnou detekci nosičů reciproké translokace. (cs)
Title
  • Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting
  • Identifikace nové reciproké translokace Y t(2;4)(q45;q34) u býka of Ayrshirského plemene využitím analýzy synaptonemálních komplexů a paintingových sond (cs)
  • Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting (en)
skos:prefLabel
  • Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting
  • Identifikace nové reciproké translokace Y t(2;4)(q45;q34) u býka of Ayrshirského plemene využitím analýzy synaptonemálních komplexů a paintingových sond (cs)
  • Identification of a new reciprocal translocation Y t(2;4)(q45;q34) in an AI bull of Ayrshire breed, with the use of synaptonemal complex technique and chromosome painting (en)
skos:notation
  • RIV/00027162:_____/08:#0000406!RIV09-MZE-00027162
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 3-4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 371197
http://linked.open...ai/riv/idVysledku
  • RIV/00027162:_____/08:#0000406
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • translocation; synaptonemal complex; chromosome painting; bull (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV
  • [92598AFE3F28]
http://linked.open...i/riv/nazevZdroje
  • Cytogenetic and Genome Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 121
http://linked.open...iv/tvurceVysledku
  • Rubeš, Jiří
  • Černohorská, Halina
  • Andersson, M.
  • Kopp, C.
  • Nowacka-Woszuk, J.
  • Sosnowski, J.
  • Switonski, M.
  • Szczerbal, I.
http://linked.open...ain/vavai/riv/wos
  • 000259032000011
http://linked.open...n/vavai/riv/zamer
issn
  • 1424-8581
number of pages
is http://linked.open...avai/riv/vysledek of
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