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| rdf:type
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| Description
| - Out of 440 bulk tank milk samples from 298 dairy herds, 70 proved positive for S. aureus (15.9%). Staphylococcal enterotoxin genes (ses) were detected in 39 (55.7%) isolates. The genes most commonly detected were sei (38.6%), seg (31.4%) and sea (27.1%). Genes seb, seh, sed, sej and sec were observed in 10%, 4.3%, 2.9%, 2.9% and 1.4% of strains respectively. Genes see and sel did not occur. The most frequently detected genotypes were seg, sei at 11.4%; sea at 10.0%; and sea, seg, sei at 8.6%. Toxin production was observed in nine (12.9%) S. aureus isolates. Seven strains were detected as SEB- (10%) and two as SED- (2.9%) producing. A relatively high number (32%) of discrepancies between the results with multiplex PCR and RPLA assays was obtained, particularly on account of SEA. The results of both methods were identical concerning SEB and SED. It was concluded that detection of ses by PCR was a useful additional tool to support identification of enterotoxigenic strains.
- Out of 440 bulk tank milk samples from 298 dairy herds, 70 proved positive for S. aureus (15.9%). Staphylococcal enterotoxin genes (ses) were detected in 39 (55.7%) isolates. The genes most commonly detected were sei (38.6%), seg (31.4%) and sea (27.1%). Genes seb, seh, sed, sej and sec were observed in 10%, 4.3%, 2.9%, 2.9% and 1.4% of strains respectively. Genes see and sel did not occur. The most frequently detected genotypes were seg, sei at 11.4%; sea at 10.0%; and sea, seg, sei at 8.6%. Toxin production was observed in nine (12.9%) S. aureus isolates. Seven strains were detected as SEB- (10%) and two as SED- (2.9%) producing. A relatively high number (32%) of discrepancies between the results with multiplex PCR and RPLA assays was obtained, particularly on account of SEA. The results of both methods were identical concerning SEB and SED. It was concluded that detection of ses by PCR was a useful additional tool to support identification of enterotoxigenic strains. (en)
- Bylo vyšetřeno 440 bazénových vzorků mléka z 298 mléčných stád, z nichž 70 vzorků bylo pozitivních na přítomnost S. aureus (15,9 %). Geny kódující stafylokokové enterotoxiny (ses)byly detekovány u 39 (55,7 %) izolátů. Nejčastěji detekované byly geny sei (38,6 %), seg (31,4 %) a sea (27,1 %). Geny seb, seh, sed, sej a sec byly pozorovány v 10 %; 4,3 %, 2,9 %, 2,9 % a 1,4 % případů. Geny see a sel nebyly zachyceny. Nejčastějším genotypem byl seg, sei (11,4 %); sea (10 %); a sea, seg, sei (8,6 %). Produkce toxinů byla pozorována u 9 (12,9 %) izolátů. SEB produkovalo 7 kmenů (10 %), SED 2 kmeny (2,9 %). Ukázaly se poměrně značné diskrepance mezi oběma metodami, zejména díky SEA. Naopak výsledky obou metod se shodovaly v případech SEB a SED. Detekce ses metodou PCR je užitečnou doplňkovou metodou pro identifikaci enterotoxigenních kmenů. (cs)
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| Title
| - Multiplex PCR and RPLA Identification of Staphylococcus aureus Enterotoxigenic Strains From Bulk Tank Milk
- Multiplex PCR and RPLA Identification of Staphylococcus aureus Enterotoxigenic Strains From Bulk Tank Milk (en)
- Identifikace enterotoxigenních kmenů Staphylococcus aureus v bazénových vzorcích mléka metodami multiplex PCR a RPLA (cs)
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| skos:prefLabel
| - Multiplex PCR and RPLA Identification of Staphylococcus aureus Enterotoxigenic Strains From Bulk Tank Milk
- Multiplex PCR and RPLA Identification of Staphylococcus aureus Enterotoxigenic Strains From Bulk Tank Milk (en)
- Identifikace enterotoxigenních kmenů Staphylococcus aureus v bazénových vzorcích mléka metodami multiplex PCR a RPLA (cs)
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| skos:notation
| - RIV/00027162:_____/08:#0000346!RIV09-MZE-00027162
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/08:#0000346
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - multiplex PCR; reversed passive latex agglutination; dairy cow (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Zoonoses and Public Health
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Ryšánek, Dušan
- Zouharová, Monika
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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