| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - V průběhu posledních let je pozorován zvýšený zájem o rychlé visuální testy, které by mohli být prováděny mimo laboratoř, např. na farmách nebo výrobních podnicích. Zde je popsána jednoduchá a na přístroji nazávislá dipstick immunoassay technika pro detekci sulfonamidů. Polyklonální protilátka byla optimalizována vzhledem k potažení na nitrocelulosové membráně, ředění peroxidásového konjugátu, blokačního činidla a času inkubace. Výsledky získané visuálním metodou mohou být získány v průběhu 20 minut. Sulfamethazinem fortifikované vzorky vody odstředěného mléka a extraktu prasečí kejdy o koncentraci 50 and 100 ug/l, vykázaly jasné visuální odlišení (nižší intensita zbarvení) ve srovnání s s kontrolními vzorky (vysoká intensita) v dipstick systému. (cs)
- During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. A simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase conjugate, blocking agents and incubation times. Test results assessed by visual measurement can be available within 20 minutes. In buffer, water, skimmed milk and pig manure extract, sulfamethazine fortified at 50 and 100 ug/l has exhibited clear visual differentiation in colour development (lower intensity) in comparison to the control spot intensity (high intensity) of the dipstick.
- During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. A simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase conjugate, blocking agents and incubation times. Test results assessed by visual measurement can be available within 20 minutes. In buffer, water, skimmed milk and pig manure extract, sulfamethazine fortified at 50 and 100 ug/l has exhibited clear visual differentiation in colour development (lower intensity) in comparison to the control spot intensity (high intensity) of the dipstick. (en)
|
| Title
| - Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay
- Detekce sulfamethazinu ve vodě, mléce a prasečí kejdě s použitím dipstick immunoanalýzy (cs)
- Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay (en)
|
| skos:prefLabel
| - Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay
- Detekce sulfamethazinu ve vodě, mléce a prasečí kejdě s použitím dipstick immunoanalýzy (cs)
- Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay (en)
|
| skos:notation
| - RIV/00027162:_____/07:#0000254!RIV08-MZE-00027162
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/07:#0000254
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - sulfonamides; polyclonal antibody; membrane; immunoassay; on-site analysis (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Hruška, Karel
- Fránek, Milan
- Kandimalla, Vivek Babu
- Kandimalla, Nagamani
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| is http://linked.open...avai/riv/vysledek
of | |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)