| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Vybrané králíčí polyklonální protilátky proti lidským antigenům byly použity jako příklad nástroje pro studie struktury a vývoje imunitního systému u veterinárních druhů. Směs F(ab')2 fragmentů protilátek proti lidskému Ig kappa-FITC/anti-Ig lambda-RPE byla použita pro dvoubarevnou a, v kombinaci s křížově reagující protilátkou HM-57 proti antigenu CD79alfa, trojbarevnou průtokovou cytometrii B lymfocytů periferní krve psa, kočky, skotu a prasete. Králičí protilátka proti lidskému laktoferinu byla použita pro vizualizaci neutrofilních granulocytů psa, prasete a skotu a byla naržena aplikace dvoubarevné imunofenotypizace psích granulocytů. Dále byla prokázána použitelnost protilátky proti CD3 a TdT pro studie lymfopoézy. Práce jako celek dokazuje, že pečlivě vybrané polyklonální protilátky dostupné na trhu mohou vykazovat širokou křížovou reaktivitu a jsou použitelné pro aplikace ve veterinárním výzkumu. (cs)
- We have used selected rabbit anti-human polyclonal antibodies as an example of useful tools for studies on immune system structure and development in veterinary species. The cocktail of anti-human Ig kappa-FITC/anti-Ig lambda-RPE F(ab')2 fragments was used for two-colour and, in combination with the cross-reactive anti-CD79alpha monoclonal antibody HM-57, for three-colour flow cytometry of canine, feline, bovine and porcine peripheral B-cells. Rabbit anti-human lactoferrin-FITC F(ab')2 fragment was used for visualizing neutrophils in dogs, pigs and cattle and an application for two-colour immunophenotyping of canine granulocyte subsets has been designed. Affinity isolated rabbit anti-human CD3 and anti-human TdT have been shown to represent a ready-to-use tool for in situ studies on primary lymphopoiesis. Altogether, our study show that carefully selected polyclonal antibodies available on the market may possess broad cross-reactivity with applications in veterinary research
- We have used selected rabbit anti-human polyclonal antibodies as an example of useful tools for studies on immune system structure and development in veterinary species. The cocktail of anti-human Ig kappa-FITC/anti-Ig lambda-RPE F(ab')2 fragments was used for two-colour and, in combination with the cross-reactive anti-CD79alpha monoclonal antibody HM-57, for three-colour flow cytometry of canine, feline, bovine and porcine peripheral B-cells. Rabbit anti-human lactoferrin-FITC F(ab')2 fragment was used for visualizing neutrophils in dogs, pigs and cattle and an application for two-colour immunophenotyping of canine granulocyte subsets has been designed. Affinity isolated rabbit anti-human CD3 and anti-human TdT have been shown to represent a ready-to-use tool for in situ studies on primary lymphopoiesis. Altogether, our study show that carefully selected polyclonal antibodies available on the market may possess broad cross-reactivity with applications in veterinary research (en)
|
| Title
| - Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species
- Komerčně dostupné králíčí polyklonální protilátky jako nástro studií imunitního systému u veterinárních druhů (cs)
- Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species (en)
|
| skos:prefLabel
| - Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species
- Komerčně dostupné králíčí polyklonální protilátky jako nástro studií imunitního systému u veterinárních druhů (cs)
- Commercially available rabbit anti-human polyclonal antisera as a useful tool for immune system studies in veterinary species (en)
|
| skos:notation
| - RIV/00027162:_____/07:#0000167!RIV08-MZE-00027162
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GP524/02/P010), Z(MZE0002716201)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/07:#0000167
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Canine; Porcine; Bovine; Feline; Lactoferrin; TdT; Immunoglobulin light chain; Flow cytometry; Immunohistochemistry (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Veterinary Immunology and Immunopathology
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Faldyna, Martin
- Levá, Lenka
- Kummer, Vladimír
- Mašková, Jarmila
- Šinkora, J.
- Řeháková, Zuzana
- Šamánková, Petra
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| is http://linked.open...avai/riv/vysledek
of | |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)