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| Description
| - Fakultativně intracelulární patogen Francisella tularensis je původce vážného infekčního onemocnění tularémie. Navzdory intenzivnímu výzkumu nejsou stále známy faktory virulence a mechanismy patogenity. Za účelem identifikace domnělých faktorů virulence jsme provedli srovnávací proteomovou analýzu frakcí obohacených o membránové proteiny izolované z vysoce virulentního poddruhu tularensis kmen SCHU S4 a třech zástupců poddruhu holarctica o různé virulenci včetně živého vakcinačního kmene. Identifikovali jsme 6 proteinů specifických pouze pro kmen SCHU S4 a 4 proteiny exprimované ve vyšší koncentraci než u kmenů poddruhu holarctica. Geny kódující tyto proteiny byly sekvenovány k potvrzení výsledků z proteomové analýzy. Naše studie naznačuje, že specifické proteiny poddruhu tularensis reprezentují potenciální faktory virulence. (cs)
- The facultative intracellular pathogen Francisella tularensis is the causative agent of the serious infectious disease tularemia. Despite intensive research, the virulence factors and pathogenetic mechanisms remain largely unknown. In order to identify novel putative virulence factors we carried out a comparative proteome analysis of fractions enriched for membrane-associated proteins isolated from the highly virulent subspecies tularensis strain SCHU S4 and three representatives of subspecies holarctica of different virulence including the live vaccine strain. We identified six proteins uniquely expressed and four proteins expressed at significantly higher levels by SCHU S4 compared to the ssp. holarctica strains. The genes encoding proteins of particular interest were examined by sequencing in order to confirm and explain the findings of the proteome analysis. Our studies suggest that the subspecies tularensis-specific proteins represent novel potential virulence factors.
- The facultative intracellular pathogen Francisella tularensis is the causative agent of the serious infectious disease tularemia. Despite intensive research, the virulence factors and pathogenetic mechanisms remain largely unknown. In order to identify novel putative virulence factors we carried out a comparative proteome analysis of fractions enriched for membrane-associated proteins isolated from the highly virulent subspecies tularensis strain SCHU S4 and three representatives of subspecies holarctica of different virulence including the live vaccine strain. We identified six proteins uniquely expressed and four proteins expressed at significantly higher levels by SCHU S4 compared to the ssp. holarctica strains. The genes encoding proteins of particular interest were examined by sequencing in order to confirm and explain the findings of the proteome analysis. Our studies suggest that the subspecies tularensis-specific proteins represent novel potential virulence factors. (en)
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| Title
| - Comparative proteome analysis of fractions enriched for membrane-associated proteins from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica strains
- Comparative proteome analysis of fractions enriched for membrane-associated proteins from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica strains (en)
- Srovnávací proteomová analýza frakcí membránových proteinů z Francisella tularensis subsp. tularensis a F. tularensis subsp. holarctica (cs)
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| skos:prefLabel
| - Comparative proteome analysis of fractions enriched for membrane-associated proteins from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica strains
- Comparative proteome analysis of fractions enriched for membrane-associated proteins from Francisella tularensis subsp. tularensis and F. tularensis subsp. holarctica strains (en)
- Srovnávací proteomová analýza frakcí membránových proteinů z Francisella tularensis subsp. tularensis a F. tularensis subsp. holarctica (cs)
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| skos:notation
| - RIV/00027162:_____/06:#0000137!RIV07-MZE-00027162
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00027162:_____/06:#0000137
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - F. tularensis, comparative proteomics, membrane proteins, virulence factors (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Proteome Research
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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