About: Isolation and partial characterization of a novel porcine astrovirus

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a dirrhoeal specimen. Furthemore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus,PAstV. Phylogenetic analysis clustered the novel isolate,Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses. Astroviral infection has been described as one of the causes of porcine diarrhoeal disease. Here we describe the detection of astrovirus-like particles by electron microscopy in a dirrhoeal specimen. Furthemore, a cytopathic virus was isolated and propagated in an established porcine kidney cell line, PK-15. Reverse transcription and PCR performed with astrovirus-specific primers amplified a product with the expected size. Sequencing of the PCR product revealed that the virus observed by electron microscopy and propagated in the porcine cell line is an astrovirus, showing 86% identity at the nucleotide level with the only known porcine astrovirus,PAstV. Phylogenetic analysis clustered the novel isolate,Sb4685, together with PAstV in a broad clade comprising mammalian astroviruses. (en) Je popsána astrovirová infekce prasat jako příčina průjmu u selat. Virus byl prokázán ve vzorku trusu z průjmujícího selete a izolován na buněčné línii PK-15. Pro průkaz astroviru byla použita metoda RT-PCR a sekvenování PCR produktu. Sekvenování prokázalo 86% identitu nukleotidů s jediným dosud známým astrovirem prasat, PAstV. Byla také provedena fylogenetická analýza nového izolátu, Sb4685. Jedná se o prioritní izolaci astroviru prasat v ČR a druhou izolaci ve světě. (cs)
Title	Isolation and partial characterization of a novel porcine astrovirus Izolace a částečná charakterizace nového astroviru prasat (cs) Isolation and partial characterization of a novel porcine astrovirus (en)
skos:prefLabel	Isolation and partial characterization of a novel porcine astrovirus Izolace a částečná charakterizace nového astroviru prasat (cs) Isolation and partial characterization of a novel porcine astrovirus (en)
skos:notation	RIV/00027162:_____/06:#0000122!RIV07-MZE-00027162
http://linked.open.../vavai/riv/strany	2-4
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QF4051), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika	2-4
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Valíček, Lubomír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	480512
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/06:#0000122
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	porcine astrovirus, isolation, electron microscopy, propagation, phylogeny (en)
http://linked.open.../riv/klicoveSlovo	electron microscopy isolation phylogeny propagation porcine astrovirus
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A83A6038D041]
http://linked.open...i/riv/nazevZdroje	Veterinary Microbiology
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	The use of biotechnological procedures in the diagnosis and immunoprophylaxis of viral gastroenteritides of swine
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Valíček, Lubomír
http://linked.open...n/vavai/riv/zamer	Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí
issn	0378-1135
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Isolation and partial characterization of a novel porcine astrovirus Isolation and partial characterization of a novel porcine astrovirus

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