About: Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.vuzv.cz/sites/File/_privat/12104.pdf
Description	The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ER alpha) and beta (ER beta) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RT-PCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ER alpha gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ER beta mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged culture of pig GC under different conditions suggests the role of oestrogens acting via their receptors in maintaining survivability of porcine granulosa cells in vitro. The aim of the present work was to determine the relative transcript abundance of the oestrogen receptor alpha (ER alpha) and beta (ER beta) gene in long-term cultures (up to 18 days) of pig granulosa cells (GC) obtained from small (1-2 mm; SF-GC) and large (5-7 mm; LF-GC) follicles. GC were cultured in the presence of 10% foetal calf serum (FCS) and one of the following growth factors: leukaemia inhibitory factor (LIF), basic fibroblast growth factor (bFGF), or stem cell factor (SCF). The relative transcript abundance of the investigated genes was measured by real time PCR (RT-PCR). In comparison with the control (medium only), LIF significantly increased (P<0.05-0.01) ER alpha gene expression in SF-GC after 3, 15 and 18 days of culture and after 12 days in LF-GC. The ER beta mRNA level in SF-GC was significantly decreased (P<0.01) by all of the investigated factors after 3, 6, 9 and 12 days of incubation. In conclusion, expression of both types of oestrogen receptors during prolonged culture of pig GC under different conditions suggests the role of oestrogens acting via their receptors in maintaining survivability of porcine granulosa cells in vitro. (en)
Title	Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells (en)
skos:prefLabel	Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells (en)
skos:notation	RIV/00027014:_____/12:#0001661!RIV13-MZE-00027014
http://linked.open...avai/predkladatel	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002701401)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Chronowska, Ewa Kott, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	163798
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/12:#0001661
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	pig, granulosa cells, oestrogen receptor, mRNA, prolonged culture, growth factors (en)
http://linked.open.../riv/klicoveSlovo	granulosa cells oestrogen receptor prolonged culture pig mRNA growth factors
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[BCE8D1B92E97]
http://linked.open...i/riv/nazevZdroje	Journal of Animal and Feed Sciences
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	21
http://linked.open...iv/tvurceVysledku	Chronowska, Ewa Kott, Tomáš
http://linked.open...ain/vavai/riv/wos	000306726400008
http://linked.open...n/vavai/riv/zamer	Od rostoucího oocytu k produkci embryí in vitro a embryonálním kmenovým buňkám: Vývojové modely a strategie produkce zárodečných, embryonálních a somatických buněk in vitro v kontextu s jejich využitím v programu zachování genetických živočišných zdrojů
issn	1230-1388
number of pages	11 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Quantitative analysis of oestrogen receptor alpha and beta gene expression during prolonged culture of pig granulosa cells

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