About: Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.vuzv.cz/sites/File/_privat/12075.pdf
Description	Genetic polymorphisms of bovine κ-casein (CSN3) and β-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR–RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses. Moreover, it is a cheap and fast alternative for existing methods. Genetic polymorphisms of bovine κ-casein (CSN3) and β-lactoglobulin (LGB) have been shown to affect milk compositional traits and technological properties. Monitoring individual CSN3 and LGB genetic variants in the population is important in the subsequent process of breeding cattle and improving milk protein quality. The objective of this study was to develop and validate a method for genotyping the main mutations of the bovine CSN3 and LGB genes using real time PCR (RT-PCR) followed by a high-resolution melting method (HRM) assay. The HRM-based assay allowed the sensitive and fast identification of the CSN3 AA, AB, BB, AE and BE genotypes and LGB AA, AB and BB genotypes simultaneously. This method was validated by analyzing 100 Fleckvieh cattle DNA samples, which were also genotyped by conventional methods at the molecular level using PCR–RFLP and DNA sequencing techniques. The genotypes obtained using the HRM approach perfectly matched the genotypes obtained by conventional analyses. Moreover, it is a cheap and fast alternative for existing methods. (en)
Title	Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis (en)
skos:prefLabel	Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis (en)
skos:notation	RIV/00027014:_____/12:#0001634!RIV13-MZE-00027014
http://linked.open...avai/predkladatel	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZE0002701404)
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Czerneková, Vladimíra Sztankóová, Zuzana Rychtářová, Jana Kyselová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	168110
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/12:#0001634
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cattle, CSN3, LGB, Genotyping, High-resolution melting (en)
http://linked.open.../riv/klicoveSlovo	Cattle CSN3 High-resolution melting LGB Genotyping
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[29AAF7F5AEF9]
http://linked.open...i/riv/nazevZdroje	Livestock Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	145
http://linked.open...iv/tvurceVysledku	Czerneková, Vladimíra Sztankóová, Zuzana Kyselová, Jitka Rychtářová, Jana
http://linked.open...ain/vavai/riv/wos	000303270400037
http://linked.open...n/vavai/riv/zamer	Udržitelný rozvoj chovu hospodářských zvířat v evropském modelu multifunkčního zemědělství
issn	1871-1413
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.livsci.2011.12.018
is http://linked.open...avai/riv/vysledek of	Simultaneous identification of CSN3 and LGB genotypes in cattle by high-resolution melting curve analysis

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