About: Production of giant mouse oocyte nucleoli and assessment of their protein content

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Production of giant mouse oocyte nucleoli and assessment of their protein content Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.vuzv.cz/sites/File/_privat/12068.pdf
Description	Compared with advanced developmental stage embryos and somatic cells, fully grown mammalian oocytes contain specific nucleolus-like structures (NPB - nucleolus precursor bodies). It is commonly accepted that they serve as a store of material(s) from which typical nucleoli are gradually formed. Whilst nucleoli from somatic cells can be collected relatively easily for further biochemical analyses, a sufficient number of oocyte nucleoli is very difficult to obtain. We have found that isolated oocytes nucleoli fuse very efficiently when contact is established between them. Thus, well visible giant nucleoli can be obtained, relatively easily handled and then used for further biochemical analyses. With the use of colloidal gold staining, we estimated that a single fully grown mouse oocyte nucleolus contains approximately 1.6 ng of protein. We do believe that this approach will accelerate further research aiming at analyzing the composition of oocyte nucleoli in more detail. Compared with advanced developmental stage embryos and somatic cells, fully grown mammalian oocytes contain specific nucleolus-like structures (NPB - nucleolus precursor bodies). It is commonly accepted that they serve as a store of material(s) from which typical nucleoli are gradually formed. Whilst nucleoli from somatic cells can be collected relatively easily for further biochemical analyses, a sufficient number of oocyte nucleoli is very difficult to obtain. We have found that isolated oocytes nucleoli fuse very efficiently when contact is established between them. Thus, well visible giant nucleoli can be obtained, relatively easily handled and then used for further biochemical analyses. With the use of colloidal gold staining, we estimated that a single fully grown mouse oocyte nucleolus contains approximately 1.6 ng of protein. We do believe that this approach will accelerate further research aiming at analyzing the composition of oocyte nucleoli in more detail. (en)
Title	Production of giant mouse oocyte nucleoli and assessment of their protein content Production of giant mouse oocyte nucleoli and assessment of their protein content (en)
skos:prefLabel	Production of giant mouse oocyte nucleoli and assessment of their protein content Production of giant mouse oocyte nucleoli and assessment of their protein content (en)
skos:notation	RIV/00027014:_____/12:#0001626!RIV13-GA0-00027014
http://linked.open...avai/predkladatel	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GPP302/11/P069)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Fulka, Josef Fulková, Helena Martínková, Stanislava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	162466
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/12:#0001626
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Fusion, Nucleolus, Oocyte, Protein content (en)
http://linked.open.../riv/klicoveSlovo	Oocyte Nucleolus Protein content Fusion
http://linked.open...odStatuVydavatele	JP - Japonsko
http://linked.open...ontrolniKodProRIV	[33619F453451]
http://linked.open...i/riv/nazevZdroje	Journal of Reproduction and Development
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of the nucleolus precursor body in oogenesis and early embryonic development
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	58
http://linked.open...iv/tvurceVysledku	Fulka, Josef Langerová, Alena Fulková, Helena Kyogoku, Hirohisa Martínková, Stanislava
http://linked.open...ain/vavai/riv/wos	000305866300016
issn	0916-8818
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Production of giant mouse oocyte nucleoli and assessment of their protein content

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software