About: Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis

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About: Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The advantage of LightCycler analysis (LCA) is based on speed, efficiency, and safety (absence of hazardous chemicals). At present, work is in progress to analyze further polymorphisms within CSN2 locus to allow the simultaneous detection of all the known genetic variants of the gene and exploit all the potential of the LCA technique. In this study, a protocol was developed for rapid genotyping of A and C variants at the CSN2 locus in two goat breeds reared in the Czech Republic validated (White Shorthaired and Brown Shorthaired goat). Monitoring of CSN2 variability in the goat breeds indicated the predominance of the C allele. In both breeds, CSN2A and CSN2C showed almost similar frequencies. The advantage of LightCycler analysis (LCA) is based on speed, efficiency, and safety (absence of hazardous chemicals). At present, work is in progress to analyze further polymorphisms within CSN2 locus to allow the simultaneous detection of all the known genetic variants of the gene and exploit all the potential of the LCA technique. In this study, a protocol was developed for rapid genotyping of A and C variants at the CSN2 locus in two goat breeds reared in the Czech Republic validated (White Shorthaired and Brown Shorthaired goat). Monitoring of CSN2 variability in the goat breeds indicated the predominance of the C allele. In both breeds, CSN2A and CSN2C showed almost similar frequencies. (en) Výhodami analýzy přístrojem LightCycler (LCA) jsou rychlost, efektivnost a bezpečnost (absence nebezpečných chemikálií). V současnosti probíhají práce na analýze dalších polymorfismů na lokusu CSN2 s cílem simultánní detekce všech známých genetických variant genu a využití všech možností techniky LCA. V této práci byl vytvořen protokol umožňující rychlou genotypizaci variant A a C lokusu CSN2 u plemen koz chovaných v České republice (bílá krátkosrstá a hnědá krátkosrstá). Monitorováním variability CSN2 u těchto plemen koz se zjistila převaha alely C. U obou plemen vykázaly varianty CSN2A a CSN2C téměř shodné frekvence. (cs)
Title	Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis (en) Technická poznámka: Detekce alely C beta kaseinu (CSN2) u českých plemen dojných koz analýzou na přístroji LightCycler (cs)
skos:prefLabel	Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis (en) Technická poznámka: Detekce alely C beta kaseinu (CSN2) u českých plemen dojných koz analýzou na přístroji LightCycler (cs)
skos:notation	RIV/00027014:_____/08:#0000866!RIV09-MZE-00027014
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD523/03/H076), Z(MZE0002701401)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Kottová, Eva Kott, Tomáš Sztankóová, Zuzana Kyselová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav živočišné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	399122
http://linked.open...ai/riv/idVysledku	RIV/00027014:_____/08:#0000866
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	goat; CSN2; genetic polymorphism; LightCycler analysis (en)
http://linked.open.../riv/klicoveSlovo	CSN2 LightCycler analysis goat genetic polymorphism
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[9D07AC97712F]
http://linked.open...i/riv/nazevZdroje	Journal of Dairy Science
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Enhancing of Methodological Level and Theoretical Education of Students of the Ph. D. Program 4103V - Animal Husbandry - a Perspective Field General Animal Husb
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	91
http://linked.open...iv/tvurceVysledku	Kott, Tomáš Sztankóová, Zuzana Kyselová, Jitka Kottová, Eva
http://linked.open...ain/vavai/riv/wos	000259703600039
http://linked.open...n/vavai/riv/zamer	Od rostoucího oocytu k produkci embryí in vitro a embryonálním kmenovým buňkám: Vývojové modely a strategie produkce zárodečných, embryonálních a somatických buněk in vitro v kontextu s jejich využitím v programu zachování genetických živočišných zdrojů
issn	0022-0302
number of pages	5 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis Technical note: Detection of the C allele of beta-casein (CSN2) in Czech dairy goat breeds using LightCycler analysis

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