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  • In our experiments we have tested the viability of freeze-dried somatic cell nuclei after their injection into enucleated sheep oocytes. Several modifications of lyophilisation were developed. We have demonstrated that approximately 16% of reconstructed embryos with lyophilised somatic cell nuclei developed at least up to blastocyst stage.
  • In our experiments we have tested the viability of freeze-dried somatic cell nuclei after their injection into enucleated sheep oocytes. Several modifications of lyophilisation were developed. We have demonstrated that approximately 16% of reconstructed embryos with lyophilised somatic cell nuclei developed at least up to blastocyst stage. (en)
  • V našich pokusech jsme testovali viabilitu jader somatických buněk, které byly předtím uchovány s pomocí metody lyofilizace. Bylo použito několik postupů lyofilizace. Výsledky dokázaly, že přinejmenším 16% rekonstruovaných embryí se vyvíjí až do stádia blastocysty. (cs)
Title
  • Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes
  • Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes (en)
  • Přenosy jader lyofilizovaných somatických buněk do enukleovaných oocytů ovcí (cs)
skos:prefLabel
  • Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes
  • Nuclear transfer of freeze-dried somatic cells into enucleated sheep oocytes (en)
  • Přenosy jader lyofilizovaných somatických buněk do enukleovaných oocytů ovcí (cs)
skos:notation
  • RIV/00027014:_____/08:#0000819!RIV09-MZE-00027014
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002701401)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 383406
http://linked.open...ai/riv/idVysledku
  • RIV/00027014:_____/08:#0000819
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • nuclear transfer; somatic cells; reprogramming (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [8689B994B37E]
http://linked.open...i/riv/nazevZdroje
  • Reproduction in Domestic Animals
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 43
http://linked.open...iv/tvurceVysledku
  • Fulka, Josef
  • Loi, P.
http://linked.open...ain/vavai/riv/wos
  • 000257700600058
http://linked.open...n/vavai/riv/zamer
issn
  • 0936-6768
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