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  • The aim of this study was to utilize the lactulose/mannitol test for assessment of intestinal permeability (IP) in preruminant calves. IP was increased experimentally by administration of Indomethacin. Both sugar markers were determined simultaneously as silyl-derivates in 5 h urinary production using the gas liquid chromatography technique. The index of IP was determined as the ratio between urinary recovery (%) lactulose and D-mannitol. The IP index in control calves was 0.2421. Results of this study show that ratio of lactulose to D-mannitol in urine reflected the treatment by the highest dose of Indomethacin and that the test may be used for determination of IP in preruminant calves.
  • The aim of this study was to utilize the lactulose/mannitol test for assessment of intestinal permeability (IP) in preruminant calves. IP was increased experimentally by administration of Indomethacin. Both sugar markers were determined simultaneously as silyl-derivates in 5 h urinary production using the gas liquid chromatography technique. The index of IP was determined as the ratio between urinary recovery (%) lactulose and D-mannitol. The IP index in control calves was 0.2421. Results of this study show that ratio of lactulose to D-mannitol in urine reflected the treatment by the highest dose of Indomethacin and that the test may be used for determination of IP in preruminant calves. (en)
  • Cílem této práce bylo využít laktulózo-mannitolový test pro stanovení střevní propustnosti (SP) u telat v období mléčné výživy. SP byla pokusně zvýšena aplikací Indomethacinu (I). Z 5 hodinové produkce moči byly stanoveny simultánně. Oba sacharidové markery byly stanoveny v 5ti hodinovém odpadu moči po předchozí derivatizaci na silyl-estery. Ke stanovení byla použita metoda GLC. Index SP vyjádřený jako poměr mezi zpětně vyloučenou laktulózou a D-mannitolem (%) byl u kontrolních telat (bez aplikace I) 0,242. Výsledky studie ukázaly, že poměr laktulózy k D-mannitolu v moči byl nejvyšší dávkou Indomethacinu průkazně ovlivněn a tedy že je možné tímto testem stanovit IP u neruminujících telat. (cs)
Title
  • Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test
  • Stanovení střevní propustnosti u telat v období mléčné výživy laktulózo-mannitolovým testem (cs)
  • Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test (en)
skos:prefLabel
  • Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test
  • Stanovení střevní propustnosti u telat v období mléčné výživy laktulózo-mannitolovým testem (cs)
  • Assessment of intestinal permeability in preruminant calves by lactulose/mannitol test (en)
skos:notation
  • RIV/00027014:_____/07:#0000385!RIV08-MZE-00027014
http://linked.open.../vavai/riv/strany
  • 43-52
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6046137305), Z(MZE0002701403)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 410863
http://linked.open...ai/riv/idVysledku
  • RIV/00027014:_____/07:#0000385
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • intestinal permeability; calves; lactulose/mannitol test; gas liquid chromatography; Indomethacin (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • PL - Polská republika
http://linked.open...ontrolniKodProRIV
  • [2D052F26A7D9]
http://linked.open...i/riv/nazevZdroje
  • Journal of Animal and Feed Sciences
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 16
http://linked.open...iv/tvurceVysledku
  • Volek, Zdeněk
  • Klein, Pavel
  • Skřivanová, Věra
  • Kleinová, Tereza
http://linked.open...n/vavai/riv/zamer
issn
  • 1230-1388
number of pages
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