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  • A total of 93 blastocysts obtained by nuclear transfer of somatic cells (granulosa cells) were transferred into 41 recipient ewes, and pregnancies were monitored by ultrasound scanning. In vitro derived, fertilized embryos (IVF, n=123) were also transferred to assess oocyte competence, and naturally mated ewes (n=120) were analysed as well. Cloned embryos developed to the blastocysts stage and implanted at the same rate as IVF embryos. After day 30, dramatic losses occurred, and only 12 out of 93 (13%) clones reached full term development, compared to 51 out of 123 (41,6%) lamb born from the IVF control embryos.Detailed analysis showed a wide range of placental abnormalities as well as some serious abnormalities in cloned born.
  • A total of 93 blastocysts obtained by nuclear transfer of somatic cells (granulosa cells) were transferred into 41 recipient ewes, and pregnancies were monitored by ultrasound scanning. In vitro derived, fertilized embryos (IVF, n=123) were also transferred to assess oocyte competence, and naturally mated ewes (n=120) were analysed as well. Cloned embryos developed to the blastocysts stage and implanted at the same rate as IVF embryos. After day 30, dramatic losses occurred, and only 12 out of 93 (13%) clones reached full term development, compared to 51 out of 123 (41,6%) lamb born from the IVF control embryos.Detailed analysis showed a wide range of placental abnormalities as well as some serious abnormalities in cloned born. (en)
  • Do 41 ovcí (příjemkyň) bylo vpraveno 93 blastocyst, získaných transferem jádra somatických buněk (granulózní buňky) a březost bahnic byla monitorována ultrazvukem. Pro vyhodnocení kompetence oocytů byl proveden transfer embryí získaných oplodněním in vitro (IVF, n=123). Do hodnocení byly zařazeny rovněž bahnice oplodněné přirozeným způsobem (n=120). Klonovaná embrya dosáhla stádia blastocysty a implantovala se do dělohy stejným způsobem jako embrya IVF. Po 30. dni březosti docházelo k obrovským ztrátám - jen 12 klonů z 93 (13%) dospělo do konečné fáze vývoje - v porovnání s kontrolní skupinou (IVF), kde se narodilo 51 jehňat ze 123 embryí (41,6%). Detailní analýzou bylo zjištěno velké množství placentálních abnormalit i některé vážné vady u narozených klonů. (cs)
Title
  • Placental abnormalities associated with post-natal mortality in sheep somatic cell clones
  • Placental abnormalities associated with post-natal mortality in sheep somatic cell clones (en)
  • Abnormality placenty související s postnatální mortalitou klonů ze somatických buněk ovcí (cs)
skos:prefLabel
  • Placental abnormalities associated with post-natal mortality in sheep somatic cell clones
  • Placental abnormalities associated with post-natal mortality in sheep somatic cell clones (en)
  • Abnormality placenty související s postnatální mortalitou klonů ze somatických buněk ovcí (cs)
skos:notation
  • RIV/00027014:_____/06:#0000235!RIV07-MZE-00027014
http://linked.open.../vavai/riv/strany
  • 6
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002701401)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 492195
http://linked.open...ai/riv/idVysledku
  • RIV/00027014:_____/06:#0000235
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cloning, post-natal mortality, placental abnormalities, lambs (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [B0C918A41ABE]
http://linked.open...i/riv/nazevZdroje
  • Theriogenology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Vacková, Irena
  • FulkaJr., Josef
http://linked.open...n/vavai/riv/zamer
issn
  • 0093-691X
number of pages
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