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Description
| - The susceptibility of Salmonella spp. to 15 fatty acids was determined in vitro in cultures grown on glucose. Antimicrobial activity was expressed as IC50 (a concentration at which only 50% of the initial glucose in cultures was utilized). Caprylic acid was the only acid inhibiting glucose utilization. In cultures of S. enteritidis, S. infantis and S. typhimurium, IC50 of caprylic acid ranged from 0.75 to 1.17 mg/mL. A moderate adaptation effect was observed as these values increased 1.5-1.8 times when bacteria were subcultured 10 times in media containing a low concentration of caprylic acid (1/3 IC50). No effect of calcium ions added in excess on antimicrobial activity of caprylic acid was observed. Incubation of salmonellas with caprylic acid (1 mg/mL; 30 min) at pH 5.2-5.3 led to a reduction in the concentration of viable cells below the detection limit; 2-6% of Salmonella cells survived at Ph 6.3-6.6.
- The susceptibility of Salmonella spp. to 15 fatty acids was determined in vitro in cultures grown on glucose. Antimicrobial activity was expressed as IC50 (a concentration at which only 50% of the initial glucose in cultures was utilized). Caprylic acid was the only acid inhibiting glucose utilization. In cultures of S. enteritidis, S. infantis and S. typhimurium, IC50 of caprylic acid ranged from 0.75 to 1.17 mg/mL. A moderate adaptation effect was observed as these values increased 1.5-1.8 times when bacteria were subcultured 10 times in media containing a low concentration of caprylic acid (1/3 IC50). No effect of calcium ions added in excess on antimicrobial activity of caprylic acid was observed. Incubation of salmonellas with caprylic acid (1 mg/mL; 30 min) at pH 5.2-5.3 led to a reduction in the concentration of viable cells below the detection limit; 2-6% of Salmonella cells survived at Ph 6.3-6.6. (en)
- Byla určena citlivost Salmonelly spp. na 15 mastných kyselin in vitro v kulturách vypěstovaných na glukóze. Antimikrobiální aktivita byla vyjádřena jako IC50 (koncentrace, ve které bylo v kulturách spotřebováno jen 50% původní glukózy). Kaprylová kyselina byla jediná kyselina, která potlačovala využití glukózy. V kulturách S. enteritidis, S. infantis a S. typhimurium se IC50 kaprylové kyseliny pohybovalo v rozmezí od 0,75 do 1,17 mg/ml. Byl pozorován mírný adaptační efekt - hodnoty 1,5-1,8x vyšší i tehdy, když byly baktérie 10 x přeneseny do substrátu obsahujícího nízkou koncentraci kyseliny kaprylové (1/3 IC50). Vliv přidaných kalciových iontů na antimikrobiální aktivitu kaprylové kyseliny nebyl pozorován. Inkubace salmonel s kaprylovou kyselinou (1mg/ml; 30 min) při pH 5,2-5,3 vedlo k redukci koncentrace životaschopných buněk pod úroveň rozlišení; 2-6% buněk Salmonell přežívá při pH 6,3-6,6. (cs)
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Title
| - In Vitro Effect of C2 - C18 Fatty acids on Salmonellas
- In Vitro Effect of C2 - C18 Fatty acids on Salmonellas (en)
- Vliv C2 - C18 mastných kyselin na Salmonelly in vitro (cs)
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skos:prefLabel
| - In Vitro Effect of C2 - C18 Fatty acids on Salmonellas
- In Vitro Effect of C2 - C18 Fatty acids on Salmonellas (en)
- Vliv C2 - C18 mastných kyselin na Salmonelly in vitro (cs)
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skos:notation
| - RIV/00027014:_____/04:00011172!RIV/2005/GA0/M02005/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/00027014:_____/04:00011172
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - in vitro;fatty acids;salmonellas (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Marounek, Milan
- Skřivanová, Eva
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issn
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number of pages
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