About: Evaluation of wheat (Triticum aestivum L.) cultivars with standard Gli/Gla alleles using PCR and leaf tissue as a direct template.

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About: Evaluation of wheat (Triticum aestivum L.) cultivars with standard Gli/Gla alleles using PCR and leaf tissue as a direct template. Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Preliminary studies were performed to prove, whether sequence-based primers could be used for gliadin/glutenin leci identification instead of polyacrylamide or starch electrophoresis, The possibility of evaluation of DNA-based markers using direct temp late were studied showing that a segment of tissue is usable for PCR reaction in wheat tissue. Different sets of primers specific for gliadin/LMW glutenin loci were used to verify the reliability of the reaction. Segment of leaf tissue is suitable, unex oensive template, whereas coleoptile did not work for this purposes. Two sets of primers derived from gamma-gliadin pseudogne were used to analyse a set of wheat cultivars with standard gliadin alleles. Using F1xR and F2xR primer combinations, 13 differ ent band were recorded, which made differentiation of 12 groups possible. Such method could be therefore a simple and alternative method for their verification. Preliminary studies were performed to prove, whether sequence-based primers could be used for gliadin/glutenin leci identification instead of polyacrylamide or starch electrophoresis, The possibility of evaluation of DNA-based markers using direct temp late were studied showing that a segment of tissue is usable for PCR reaction in wheat tissue. Different sets of primers specific for gliadin/LMW glutenin loci were used to verify the reliability of the reaction. Segment of leaf tissue is suitable, unex oensive template, whereas coleoptile did not work for this purposes. Two sets of primers derived from gamma-gliadin pseudogne were used to analyse a set of wheat cultivars with standard gliadin alleles. Using F1xR and F2xR primer combinations, 13 differ ent band were recorded, which made differentiation of 12 groups possible. Such method could be therefore a simple and alternative method for their verification. (en)
Title	Evaluation of wheat (Triticum aestivum L.) cultivars with standard Gli/Gla alleles using PCR and leaf tissue as a direct template. Evaluation of wheat (Triticum aestivum L.) cultivars with standard Gli/Gla alleles using PCR and leaf tissue as a direct template. (en)
skos:prefLabel	Evaluation of wheat (Triticum aestivum L.) cultivars with standard Gli/Gla alleles using PCR and leaf tissue as a direct template. Evaluation of wheat (Triticum aestivum L.) cultivars with standard Gli/Gla alleles using PCR and leaf tissue as a direct template. (en)
skos:notation	RIV/00027006:_____/97:00000027!RIV/2000/GA0/M01000
http://linked.open.../vavai/riv/strany	251
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA521/97/0222)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2000
http://linked.open...aciTvurceVysledku	Kučera, Ladislav Ovesná, Jaroslava Martynková, Renata
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	797933
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/97:00000027
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PCR; glutenins; gliadins; direct template (en)
http://linked.open.../riv/klicoveSlovo	PCR gliadins glutenins direct template
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[6EF51553080B]
http://linked.open...i/riv/nazevZdroje	Genetika a Šlechtění
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular analysis of gliadin and glutenin genes in wheat and relative species
http://linked.open...UplatneniVysledku	1997
http://linked.open...v/svazekPeriodika	33
http://linked.open...iv/tvurceVysledku	Kučera, Ladislav Ovesná, Jaroslava Martynková, Renata
issn	1212-1975
number of pages	10 (xsd:int)

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