About: PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae)     Goto   Sponge   NotDistinct   Permalink

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Description
  • A specific primer set, RetCO1F/RetCO/1R was designet by targeting consensus sequences from multiple aligments of the CO1 gene Lepinotus reticulatus for use in end point PCR, SYBR Green qPCR and HDA. Lepinotus a nd Liposcelis species were tested to confirm the primer specificity. The described primer set allowed accurate identification of L. reticulatus, producing an amplicon of 119 bp in the three described assays.
  • A specific primer set, RetCO1F/RetCO/1R was designet by targeting consensus sequences from multiple aligments of the CO1 gene Lepinotus reticulatus for use in end point PCR, SYBR Green qPCR and HDA. Lepinotus a nd Liposcelis species were tested to confirm the primer specificity. The described primer set allowed accurate identification of L. reticulatus, producing an amplicon of 119 bp in the three described assays. (en)
Title
  • PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae)
  • PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae) (en)
skos:prefLabel
  • PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae)
  • PCR and isothermal-based molecular identification of the stored-product psocid pest Lepinotus reticulatus (Psocoptera: Trogiidae) (en)
skos:notation
  • RIV/00027006:_____/12:00002159!RIV13-MZE-00027006
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika
  • April
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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http://linked.open...dnocenehoVysledku
  • 158026
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/12:00002159
http://linked.open...riv/jazykVysledku
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  • Lepinotus; Molecular diagnostics; SYBR Green qPCR; CO1; Isothermal amplification (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [FB2C6714D78E]
http://linked.open...i/riv/nazevZdroje
  • Journal of Stored Products Research
http://linked.open...in/vavai/riv/obor
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http://linked.open...v/svazekPeriodika
  • 49
http://linked.open...iv/tvurceVysledku
  • Kučerová, Zuzana
  • Stejskal, Václav
  • Li, Z.
  • Yang, Q.
  • Opit, G.
  • Arif, M.
  • Ochoa-Corona, F.
http://linked.open...ain/vavai/riv/wos
  • 000304076600005
http://linked.open...n/vavai/riv/zamer
issn
  • 0022-474X
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.jspr.2012.02.001
is http://linked.open...avai/riv/vysledek of
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