About: Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius     Goto   Sponge   NotDistinct   Permalink

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  • In this paper application of RAPDs and AFLPs for the analysis of genetic diversity in a group of 5 Smallanthus sonchifolius landraces is presented. Both methods proceed through the direct analysis of DNA, and their results were compared with the total phenolic content of each landrace and its morphological traits. Using 61 RAPD primers, 85 informative bands were identified, corresponding to 28.7% of polymorphism. In comparsion, only six selected AFLP primer pairs produced 84 informative bands, with a similar percentage of polymorphism (23.4%). Total phenolic content varied twofold among the five landraces analysed, ranging from 3.494 to 6.849 mg/g. Each type of molecular marker resolved two main groups that included the same genotypes, but with different within-group relationships among genotypes. The two groups are consistent with some phenothypic characters but they do not reflect faithfully their geographical origin.
  • In this paper application of RAPDs and AFLPs for the analysis of genetic diversity in a group of 5 Smallanthus sonchifolius landraces is presented. Both methods proceed through the direct analysis of DNA, and their results were compared with the total phenolic content of each landrace and its morphological traits. Using 61 RAPD primers, 85 informative bands were identified, corresponding to 28.7% of polymorphism. In comparsion, only six selected AFLP primer pairs produced 84 informative bands, with a similar percentage of polymorphism (23.4%). Total phenolic content varied twofold among the five landraces analysed, ranging from 3.494 to 6.849 mg/g. Each type of molecular marker resolved two main groups that included the same genotypes, but with different within-group relationships among genotypes. The two groups are consistent with some phenothypic characters but they do not reflect faithfully their geographical origin. (en)
Title
  • Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius
  • Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius (en)
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  • Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius
  • Total phenolic content, RAPDs, AFLPs and morphological traits for the analysis of variability in Smallanthus sonchifolius (en)
skos:notation
  • RIV/00027006:_____/11:00001431!RIV12-MZE-00027006
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  • 4
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  • 235577
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  • RIV/00027006:_____/11:00001431
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  • DNA fingerprinting; Genetic dissimilarity; Molecular marker; Phenolic content; Smallanthus sonchifolius; Yacon (en)
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  • NL - Nizozemsko
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  • [C34A06314542]
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  • Genetic Resources and Crop Evolution
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  • 58
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  • Ovesná, Jaroslava
  • Salava, Jaroslav
  • Milella, L.
  • Fernández, E.
  • Greco, I.
  • Martelli, G.
issn
  • 0925-9864
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s10722-010-9597-x
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