About: Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The appropriate application of quantitative real-time PCR (RT-qPCR), requires the use of reference genes whose level of expression is not affected by the test, by general physiological conditions or by inter-individual variability. The expression of seven reference genes was evaluated in tissues of 180 healthy, physiologically stressed and virus-infected cereal plants by RT-qPCR. The expression of all genes did not depend on abiotic stress conditions or virus infections. GAPDH, TUBB and 18S rRNA always ranked as the three most stable genes. On the other hand, EF1A, EIF4A, and 28S rRNA for barley and oat samples; and TUBA for wheat samples were consistently ranked as the less reliable controls. The geometric average of GAPDH, 18S rRNA and TUBB is suitable for normalisation of BYDV quantification in barley tissues. For wheat and oat samples, a combination of four genes is necessary: GAPDH, 18SrRNA, TUBB and EIF4A for wheat; and GAPDH, 18S rRNA, TUBB and TUBA for oat is recommended. The appropriate application of quantitative real-time PCR (RT-qPCR), requires the use of reference genes whose level of expression is not affected by the test, by general physiological conditions or by inter-individual variability. The expression of seven reference genes was evaluated in tissues of 180 healthy, physiologically stressed and virus-infected cereal plants by RT-qPCR. The expression of all genes did not depend on abiotic stress conditions or virus infections. GAPDH, TUBB and 18S rRNA always ranked as the three most stable genes. On the other hand, EF1A, EIF4A, and 28S rRNA for barley and oat samples; and TUBA for wheat samples were consistently ranked as the less reliable controls. The geometric average of GAPDH, 18S rRNA and TUBB is suitable for normalisation of BYDV quantification in barley tissues. For wheat and oat samples, a combination of four genes is necessary: GAPDH, 18SrRNA, TUBB and EIF4A for wheat; and GAPDH, 18S rRNA, TUBB and TUBA for oat is recommended. (en)
Title	Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR (en)
skos:prefLabel	Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR (en)
skos:notation	RIV/00027006:_____/10:00001138!RIV11-MZE-00027006
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH81269), Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	léto
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Jarošová, Jana Kumar, Jiban
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	295199
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/10:00001138
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	BYDV; RT-qPCR; reference genes; virus quantification (en)
http://linked.open.../riv/klicoveSlovo	BYDV RT-qPCR reference genes virus quantification
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[B2E1A1913F0B]
http://linked.open...i/riv/nazevZdroje	BMC Plant Biology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Diagnostic methods and control measures of viruses causing dwarf diseases and their vectors in cereal crops.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Jarošová, Jana Kumar, Jiban
http://linked.open...n/vavai/riv/zamer	Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	1471-2229
number of pages	759 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR

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