About: Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic

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About: Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	One-step RT-PCR followed by a RFLP assay was developed for the typing of Barley yellow dwarf virus (BYDV) member of Luteovirus genera. A partial coat protein (CP) gene sequencing was carried out and confirmed the RFLP analysis. Both sequencing and RFLP analysis identified the presence of 3 BYDV-PAV (including the lab isolate Blatno85), 10 BYDV-MAV and 10 BYDV-PAS isolates among the field samples. These methods revealed that PAS and MAV are more dominant than PAV in the Czech Republic. One-step RT-PCR followed by a RFLP assay was developed for the typing of Barley yellow dwarf virus (BYDV) member of Luteovirus genera. A partial coat protein (CP) gene sequencing was carried out and confirmed the RFLP analysis. Both sequencing and RFLP analysis identified the presence of 3 BYDV-PAV (including the lab isolate Blatno85), 10 BYDV-MAV and 10 BYDV-PAS isolates among the field samples. These methods revealed that PAS and MAV are more dominant than PAV in the Czech Republic. (en)
Title	Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic (en)
skos:prefLabel	Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic (en)
skos:notation	RIV/00027006:_____/09:00000920!RIV10-MZE-00027006
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH81269)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Jarošová, Jana Kumar, Jiban Gadiou, Sebastien
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav rostlinné výroby, v.v.i.
http://linked.open...dnocenehoVysledku	310721
http://linked.open...ai/riv/idVysledku	RIV/00027006:_____/09:00000920
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	one-step RT-PCR; RFLP; BYDV; PAV; PAS; MAV (en)
http://linked.open.../riv/klicoveSlovo	PAV MAV BYDV PAS RFLP one-step RT-PCR
http://linked.open...odStatuVydavatele	HU - Maďarsko
http://linked.open...ontrolniKodProRIV	[D672A60B6C74]
http://linked.open...i/riv/nazevZdroje	Cereal Research Communications
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Diagnostic methods and control measures of viruses causing dwarf diseases and their vectors in cereal crops.
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	37
http://linked.open...iv/tvurceVysledku	Gadiou, Sebastien Jarošová, Jana Kumar, Jiban Červená, G.
http://linked.open...ain/vavai/riv/wos	000273068800007
issn	0133-3720
number of pages	10 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic Discrimination of Three BYDV Species by One-step RT-PCR-RFLP and Sequence Based Methods in Cereal Plants from the Czech Republic

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