About: In vitro induction of polyploidy in yacon (Smallanthus sonchifolius)     Goto   Sponge   NotDistinct   Permalink

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  • In vitro chromosome doubling was induced in octoploid (2n = 58) yacon using oryzalin and colchicine as mitotic spindle inhibitors. The resulting ploidy level was determined by chromosome counting and flow cytometry. The greatest proportions of hexadecaploid plants (1.6%) were obtained after 48 h of 25 lM oryzalin treatment. With the colchicine treatment, only 0.42% hexadecaploid plants were detected and their survival rate was significantly lower in comparison with the oryzalin treatment. In hexadecaploid yacon, significantly higher levels of saccharides were detected (FOS 13.9 g/100 g FM, fructose 4.6 g/100 g FM and glucose 2.1 g/100 g FM) compared to the octoploid control (FOS 5.3 g/100 g FM, fructose 2.9 g/100 g FM and glucose 1.0 g/100 g FM. These results indicate that in vitro treatment of nodal segments with oryzalin solution could be an effective procedure for chromosome doubling and the polyploidy breeding can help to increase the FOS content in the tuberous roots.
  • In vitro chromosome doubling was induced in octoploid (2n = 58) yacon using oryzalin and colchicine as mitotic spindle inhibitors. The resulting ploidy level was determined by chromosome counting and flow cytometry. The greatest proportions of hexadecaploid plants (1.6%) were obtained after 48 h of 25 lM oryzalin treatment. With the colchicine treatment, only 0.42% hexadecaploid plants were detected and their survival rate was significantly lower in comparison with the oryzalin treatment. In hexadecaploid yacon, significantly higher levels of saccharides were detected (FOS 13.9 g/100 g FM, fructose 4.6 g/100 g FM and glucose 2.1 g/100 g FM) compared to the octoploid control (FOS 5.3 g/100 g FM, fructose 2.9 g/100 g FM and glucose 1.0 g/100 g FM. These results indicate that in vitro treatment of nodal segments with oryzalin solution could be an effective procedure for chromosome doubling and the polyploidy breeding can help to increase the FOS content in the tuberous roots. (en)
Title
  • In vitro induction of polyploidy in yacon (Smallanthus sonchifolius)
  • In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) (en)
skos:prefLabel
  • In vitro induction of polyploidy in yacon (Smallanthus sonchifolius)
  • In vitro induction of polyploidy in yacon (Smallanthus sonchifolius) (en)
skos:notation
  • RIV/00027006:_____/09:00000767!RIV10-MZE-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • N, S, Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 318920
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/09:00000767
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Chromosome doubling; Colchicine; Flow cytometry; Hexadecaploid; Octoploid; Oryzalin; Smallanthus; Yacon (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [69D773F15A8D]
http://linked.open...i/riv/nazevZdroje
  • Plant Cell, Tissue and Organ Culture
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 97
http://linked.open...iv/tvurceVysledku
  • Vyvadilová, Miroslava
  • Viehmannová, I.
  • Greplová, M.
  • Bechyně, M.
  • Cusimamani, F.
http://linked.open...ain/vavai/riv/wos
  • 000263799100002
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-6857
number of pages
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