About: Behaviour of transgenic Plum pox virus-resistant Prunus domestica L. clone C5 grown in the open field under a high and permanent infection pressure of the PPV-REC strain     Goto   Sponge   NotDistinct   Permalink

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  • Prunus domestica L. clone C5 was inoculated with the recombinant strain (PPV-Rec) of the Plum pox virus (PPV). Non inoculated G5 plums were used as controls. Transgenic plums infected with PPV-Rec and control plants were evaluated for six years in the open field. Mild PPV symptoms, mild diffuse spots and rings, appeared on the basal leaves the second year after inoculation. The presence of PPV was confirmed by DAS-ELISA, ISEM, and RT-PCR. A reduction of symptoms and a decrease of the relative PPV concentration, as determined by DAS-ELISA in transgenic C5 plants, were observed from the third to the fifth year after virus inoculation. These results proved resitance of plum clone C5 to the PPV-Rec.
  • Prunus domestica L. clone C5 was inoculated with the recombinant strain (PPV-Rec) of the Plum pox virus (PPV). Non inoculated G5 plums were used as controls. Transgenic plums infected with PPV-Rec and control plants were evaluated for six years in the open field. Mild PPV symptoms, mild diffuse spots and rings, appeared on the basal leaves the second year after inoculation. The presence of PPV was confirmed by DAS-ELISA, ISEM, and RT-PCR. A reduction of symptoms and a decrease of the relative PPV concentration, as determined by DAS-ELISA in transgenic C5 plants, were observed from the third to the fifth year after virus inoculation. These results proved resitance of plum clone C5 to the PPV-Rec. (en)
  • Prunus domestica L. klon C byla inokulována rekombinantním kmenem viru šarky švestky (PPV-Rec).Neinokulovanéé švestky C5 byly použity jeko kontrola. Transgenní švestky infikované PPV-Rec a kontrolní rostliny byly hodnoceny šest let v polních podmínkách. Mírné příznaky šarky se objevily druhý rok po inokulaci, přítomnost viru byla potvrzena ELISA, ISEM a RT-PCR. Příznaky i relativní koncentrace PPV od 3. do 5. roku dále slábly. Výsledky prokázaly rezistenci švestky klon C5 k PPV. (cs)
Title
  • Behaviour of transgenic Plum pox virus-resistant Prunus domestica L. clone C5 grown in the open field under a high and permanent infection pressure of the PPV-REC strain
  • Behaviour of transgenic Plum pox virus-resistant Prunus domestica L. clone C5 grown in the open field under a high and permanent infection pressure of the PPV-REC strain (en)
  • Chování transgenní, k viru šarky švestky rezistentní Prunus domestica L., klon C5 v poli pod vysokým a permanentním infekčním tlakem kmenu PPV-Rec (cs)
skos:prefLabel
  • Behaviour of transgenic Plum pox virus-resistant Prunus domestica L. clone C5 grown in the open field under a high and permanent infection pressure of the PPV-REC strain
  • Behaviour of transgenic Plum pox virus-resistant Prunus domestica L. clone C5 grown in the open field under a high and permanent infection pressure of the PPV-REC strain (en)
  • Chování transgenní, k viru šarky švestky rezistentní Prunus domestica L., klon C5 v poli pod vysokým a permanentním infekčním tlakem kmenu PPV-Rec (cs)
skos:notation
  • RIV/00027006:_____/08:00000244!RIV09-MZE-00027006
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(1B53054), Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika
  • 1 Suppl.
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 357759
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/08:00000244
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Transgenic resistance; P. domestica; resistance; PPV-Rec (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • IT - Italská republika
http://linked.open...ontrolniKodProRIV
  • [C5F2CED1FB6B]
http://linked.open...i/riv/nazevZdroje
  • Journal of Plant Pathology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 90
http://linked.open...iv/tvurceVysledku
  • Polák, Jaroslav
  • Kumar, Jiban
  • Ravelonandro, M.
  • Scorza, R.
  • Jokeš, Milan
  • Pívalová, Jitka
http://linked.open...ain/vavai/riv/wos
  • 000257453800007
http://linked.open...n/vavai/riv/zamer
issn
  • 1125-4653
number of pages
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