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  • V roce 2002 byly pozorovány příznaky virové infekce na rostlinách paprik 'OL228' vzešlých z komerčně prodávaného osiva. Infikované rostliny měly mozaiku na listech a nekrotické jizvy na plodech. Elektronovým mikroskopem byly objeveny tyčinkové virové částice délky 300 nm typické pro tobamoviry. Pomocí ELISA byl identifikován Pepper mild mottle virus (PMMoV). Biologickou charakterizací na různých odrůdách paprik byl určen patotyp P1.2. Toto je první zpráva o nálezu PMMoV v České republice. Jeho rozšíření je omezené, v hlavních produkčních oblastech pěstování paprik nebyl virus zjištěn. Byla prověřena možnost dezinfekce infikovaného osiva pomocí NaOH. (cs)
  • Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for Tobacco mosaic virus (TMV) but positive reactions with an antiserum to Pepper mild mottle virus (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype Pl.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed.
  • Symptoms of viral infection were observed on plants of pepper, cv. OL 228, raised from commercial seeds of Czech origin in a greenhouse in the year 2002. Infected plants showed mosaic or mottling on leaves, and necrotic depressions on fruits. Straight, rod-shaped viral particles of about 300 nm, indicating a tobamovirus infection, were found by electron microscope. ELISA produced negative reactions for Tobacco mosaic virus (TMV) but positive reactions with an antiserum to Pepper mild mottle virus (PMMoV). In biological characterisation using pepper cultivars with the L1, L2, L3 and L4 tobamovirus resistance genes it was found that the Czech isolate of PMMoV belongs to pathotype Pl.2. This is the first report of PMMoV in the Czech Republic. Its distribution, however, may still be limited as a survey did not reveal other infections in the main pepper producing areas. As PMMoV spreads with infected seeds, the possibility of its chemical deactivation by NaOH was tested and confirmed. (en)
Title
  • First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic
  • První nález Pepper mild mottle viru v semenech paprik produkovaných v České Republice (cs)
  • First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic (en)
skos:prefLabel
  • First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic
  • První nález Pepper mild mottle viru v semenech paprik produkovaných v České Republice (cs)
  • First report of Pepper mild mottle virus in pepper seeds produced in the Czech Republic (en)
skos:notation
  • RIV/00027006:_____/06:1275!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 34;37
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MZE0002700603)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 475935
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/06:1275
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Pepper mild mottle virus; characterisation; pathotype Pl.2; Capsicum annuum; ELISA; electron microscopy; seed transmission; virus deactivation; sodium hydroxide (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [022D930500B1]
http://linked.open...i/riv/nazevZdroje
  • Plant Protection Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 42
http://linked.open...iv/tvurceVysledku
  • Svoboda, Jiří
  • Jokeš, Milan
  • Červená, G.
  • Radová, J.
http://linked.open...n/vavai/riv/zamer
issn
  • 1212-2580
number of pages
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