About: Possibilities of Pyrenophora teres detection in barley leaf tissue     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

AttributesValues
rdf:type
Description
  • Specific polymerase chain reaction (PCR) primers were developed from AFLP bands of DNA of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed to specifically amplify DNA from P. teres f.sp. teres and allow its differentiation from P. teres f.sp. maculata, which is morphologically very similar to P. teres f.sp. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungi mycelium. The PCR assay was validated with 60 samples from 10 barley hosts originating from several regions across the Czech Republic. No cross reaction was observed with DNA of several other species like P. tritici repentis, P. graminea and Helminthosporium sativum. This method is prepared to be used to detect the pathogen from environmental samples for survey and management purposes.
  • Specific polymerase chain reaction (PCR) primers were developed from AFLP bands of DNA of Pyrenophora teres, the causal agent of net blotch on barley leaves. The primers were designed to specifically amplify DNA from P. teres f.sp. teres and allow its differentiation from P. teres f.sp. maculata, which is morphologically very similar to P. teres f.sp. teres in culture. The PCR amplification was carried out successfully from DNA extracted from fungi mycelium. The PCR assay was validated with 60 samples from 10 barley hosts originating from several regions across the Czech Republic. No cross reaction was observed with DNA of several other species like P. tritici repentis, P. graminea and Helminthosporium sativum. This method is prepared to be used to detect the pathogen from environmental samples for survey and management purposes. (en)
  • Specifická polymerázová řetězová reakce (PCR) byla vyvinuta na základě analýzy AFLP Pyrenophora teres, která způsobuje chorobu síťové listové skvrnitosti u ječmene. Primery byly navrženy tak, aby bylo možno specificky detekovat každou z forem Pyrenophora teres: Pyrenophora teres f. teres a Pyrenophora teres f. maculata. PCR reakce byly prováděny s DNA extrahovanou z mycelia. PCR byla validována amplifikací 60 vzorků z 10 odrůd ječmene uměle infikovaného oběma formami Pyrenophora teres. Žádná křížová reakce nebyla pozorována s druhy P. tritici-repentis, P. graminea a Helminthosporium sativum. Tato metoda je připravena pro detekci P. teres ve vzorcích a pro monitoring jejího výskytu. (cs)
Title
  • Possibilities of Pyrenophora teres detection in barley leaf tissue
  • Possibilities of Pyrenophora teres detection in barley leaf tissue (en)
  • Možnosti detekce Pyrenophora teres v pletivech listů ječmene (cs)
skos:prefLabel
  • Possibilities of Pyrenophora teres detection in barley leaf tissue
  • Possibilities of Pyrenophora teres detection in barley leaf tissue (en)
  • Možnosti detekce Pyrenophora teres v pletivech listů ječmene (cs)
skos:notation
  • RIV/00027006:_____/04:2423!RIV08-MZE-00027006
http://linked.open.../vavai/riv/strany
  • 705;708
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QC1361)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 580573
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/04:2423
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Pyrenophora teres; PCR (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [1FA019A3E8A6]
http://linked.open...v/mistoKonaniAkce
  • Brno
http://linked.open...i/riv/mistoVydani
  • Brno
http://linked.open...i/riv/nazevZdroje
  • Proceedings of 9-th International Barley Genetics Symposium
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Kučera, Ladislav
  • Ovesná, Jaroslava
  • Minaříková, V.
  • Leišová, Leona
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
http://purl.org/ne...btex#hasPublisher
  • Agricultural Research Institute Kromeriz
https://schema.org/isbn
  • 80-902545-9-4
Faceted Search & Find service v1.16.118 as of Jun 21 2024


Alternative Linked Data Documents: ODE     Content Formats:   [cxml] [csv]     RDF   [text] [turtle] [ld+json] [rdf+json] [rdf+xml]     ODATA   [atom+xml] [odata+json]     Microdata   [microdata+json] [html]    About   
This material is Open Knowledge   W3C Semantic Web Technology [RDF Data] Valid XHTML + RDFa
OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 112 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software