| Attributes | Values |
|---|
| rdf:type
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| Description
| - Biotyp lilku černého (Solanum nigrum 1.), který nebyl citlivý k atrazinu, byl nalezen u železniční stanice Praha-Vršovice. Citlivý biotyp byl získán z lokality Vyšehořovice (Praha-východ). Průkazný rozdíl ve vzcházivosti mezi biotypy byl při výsevu semen do hloubky 1 mm. Nejvyšší vzcházivost byla u obou biotypů z hloubky 1 mm, nejnižší z hloubky 20 mm. Citlivost rostlin byla ověřena metodou pomalé fluorescence chlorofylu, která byla ve shodě s fenotypovým hodnocením rostlin ošetřených atrazinem. Pro stanovení molekulární podstaty rezistence byla sekvencována část genu kódujícího protein Dl fotosystému II. Rezistence k atrazinu byla u biotypu lilku černého z Vršovic způsobena záměnou serinu glycinem v pozici 264 aminokyselinového řetězce proteinu Dl fotosystému II. Výsledky sekvenční analýzy byly potvrzeny štěpením produktu PCR restrikčním enzymem BfaI. Byla nalezena úplná shoda mezi rezistencí k atrazinu a přítomností mutace. Vyvinutá metoda RFLP-PCR může být použita pro rychlou detekci mutace v p (cs)
- Seeds from atrazine resistant plants of black nightshade (Solanum nigrum L.) were collected at the railway station Prague-Vršovice, seeds from susceptible plants in Vyšehořovice (Prague East district). Tests on emergence showed that in both resistant and susceptible biotypes it was highest at a seeding depth of 1 mm, and that at the same seeding depth there were statistically significant differences in emergence between the resistant and susceptible biotypes. The resistance or susceptibility to atrazine was tested by both a chlorophyll fluorescence assay and spraying with atrazine. A region of the gene encoding Dl protein of photosystem II was sequenced and compared between the resistant and susceptible biotypes. Resistance to atrazine in the S. nigrum biotype from Vršovice was conferred by a glycine for serine substitution at residue 264 of the Dl protein. In the plants of the biotypes there was excellent correspondence between the presence of the mutation and herbicide resistance. The assay based on
- Seeds from atrazine resistant plants of black nightshade (Solanum nigrum L.) were collected at the railway station Prague-Vršovice, seeds from susceptible plants in Vyšehořovice (Prague East district). Tests on emergence showed that in both resistant and susceptible biotypes it was highest at a seeding depth of 1 mm, and that at the same seeding depth there were statistically significant differences in emergence between the resistant and susceptible biotypes. The resistance or susceptibility to atrazine was tested by both a chlorophyll fluorescence assay and spraying with atrazine. A region of the gene encoding Dl protein of photosystem II was sequenced and compared between the resistant and susceptible biotypes. Resistance to atrazine in the S. nigrum biotype from Vršovice was conferred by a glycine for serine substitution at residue 264 of the Dl protein. In the plants of the biotypes there was excellent correspondence between the presence of the mutation and herbicide resistance. The assay based on (en)
|
| Title
| - The emergence of a atrazine resistant black nightside (Solanum nigrum L.) biotype and molecular basis of the resistance
- The emergence of a atrazine resistant black nightside (Solanum nigrum L.) biotype and molecular basis of the resistance (en)
- Vzcházivost biotypu lilku černého (Solanum migrum L.) rezistentního vůči atrazinu a molekulární podstata této rezistence (cs)
|
| skos:prefLabel
| - The emergence of a atrazine resistant black nightside (Solanum nigrum L.) biotype and molecular basis of the resistance
- The emergence of a atrazine resistant black nightside (Solanum nigrum L.) biotype and molecular basis of the resistance (en)
- Vzcházivost biotypu lilku černého (Solanum migrum L.) rezistentního vůči atrazinu a molekulární podstata této rezistence (cs)
|
| skos:notation
| - RIV/00027006:_____/04:00000535!RIV09-MZE-00027006
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(QC1362), Z(MZE0002700603)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/04:00000535
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - black nightside; Solanum nigrum L.; resistance; atrazine; chlorophyll fluorescence; emergence; genetic variation (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Nováková, K.
- Salava, Jaroslav
- Chodová, Daniela
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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