| Attributes | Values |
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| rdf:type
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| Description
| - Cytokinin-binding proteins (CBPs) isolated from mature grains of oat (Avena sativa L.) and wheat (Triticum aestivum L.) by acid precipitation, ion-exchange and affinity chromatography had similar characteristics, although they differed somewhat in apparent molecular weight of the native protein as determined by gel filtration (109 and 133 kDa, respectively) and subunit size as estimated by SDS-polyacrylamide gel electrophoresis (47 and 55 kDa, respectively). Highly purified oat CBP showed very weak but distinct immunochemical cross-reactivity with anti-wheat CBP IgG, indicating different immunogenic properties of the two CBPs. Nevertheless, both CBPs exhibited very similar binding of different cytokinins and were characterized by high affinity for N6-benzyladenine (BA)-type and by low affinity for zeatin-type cytokinins to both wheat and oat CBPs and by somewhat higher binding activities of oat CBP compared to wheat CBP (Kds for BA: 4.6 107 M and 6.8 107 M, respectively).
- Cytokinin-binding proteins (CBPs) isolated from mature grains of oat (Avena sativa L.) and wheat (Triticum aestivum L.) by acid precipitation, ion-exchange and affinity chromatography had similar characteristics, although they differed somewhat in apparent molecular weight of the native protein as determined by gel filtration (109 and 133 kDa, respectively) and subunit size as estimated by SDS-polyacrylamide gel electrophoresis (47 and 55 kDa, respectively). Highly purified oat CBP showed very weak but distinct immunochemical cross-reactivity with anti-wheat CBP IgG, indicating different immunogenic properties of the two CBPs. Nevertheless, both CBPs exhibited very similar binding of different cytokinins and were characterized by high affinity for N6-benzyladenine (BA)-type and by low affinity for zeatin-type cytokinins to both wheat and oat CBPs and by somewhat higher binding activities of oat CBP compared to wheat CBP (Kds for BA: 4.6 107 M and 6.8 107 M, respectively). (en)
- Vazebné bílkoviny pro cytokininy (CBPs) izolované z obilek ovsa (Avena sativa)a pšenice (Triticum aestivum)izolované srážením, iontovou a afinitní chromatografií vykazovaly podobné charakteristiky; lišily se však v molekulové hmotnosti nativní bílkoviny stanovené pomocí gelové filtrace (109 and 133 kDa) velikostí subjednotek určených pomocí SDS-PA gelové elektroforézy (47 and 55 kDa). Vysoce čistá CBP ovsa vykazovala velmi slabou, avšak odlišitelnou imunochemickou reakci s pšeničným antigenem CBP IgG, která naznačuje odlišné imunochemické vlastnosti obou CBPs. Obě CBPs vykazovaly velmi podobné vazebné vlastnosti pro různé cytokininy a byly charakterizovány vysokou afinitou pro cytokininy N6- benzyladeninového (BA) typu a nízkou afinitou pro cytokininy zeatinového typu.Vazebná aktivita CBP ovsa byla vyšší ve srovnání s vazebnou aktivitou CBP pšenice. (cs)
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| Title
| - Comparison of cytokinin-binding proteins from wheat and oat grains
- Comparison of cytokinin-binding proteins from wheat and oat grains (en)
- Porovnání vazebných bílkovin pro cytokininy z obilek ovsa a pšenice (cs)
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| skos:prefLabel
| - Comparison of cytokinin-binding proteins from wheat and oat grains
- Comparison of cytokinin-binding proteins from wheat and oat grains (en)
- Porovnání vazebných bílkovin pro cytokininy z obilek ovsa a pšenice (cs)
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| skos:notation
| - RIV/00027006:_____/03:2711!RIV08-GA0-00027006
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA522/02/0530), P(GV206/96/K188), Z(AV0Z5038910)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/00027006:_____/03:2711
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Cytokinin-binding proteins, Avena sativa, SDS-polyacrylamide gel (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - FR - Francouzská republika
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Gaudinová, Alena
- Kamínek, Miroslav
- Trčková, Marie
- Fox, Eugene J.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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