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Description
  • The encapsulation–dehydration method is used for the cryopreservation of shoot tips or meristematic tissue of vegetative propagated plants. It is supposed that the shoot tips are able to survive due to the glassy state produced during rapid freezing of plant parts to the temperature of liquid nitrogen. The glass transition temperature of encapsulated shoot tips can be controlled by varying the hydration level. Deeper dehydration increases the glass transition temperature. There is a problem with the desiccation of plants, because at a certain level of dehydration the plant cells are irreversibly injured. In these experiments we used meristem shoot tips that were approximately 2 mm long, dissected from bulblets of garlic (Allium sativum L. acc. No.1669). The meristems were kept on agar with 0.6 M sucrose in petri dishes for 24 h. Thereafter, the meristems were encapsulated in 2% alginate containing 0.7 M sucrose. The alginate beads with the meristems were desiccated over saturated salt solutions or de
  • The encapsulation–dehydration method is used for the cryopreservation of shoot tips or meristematic tissue of vegetative propagated plants. It is supposed that the shoot tips are able to survive due to the glassy state produced during rapid freezing of plant parts to the temperature of liquid nitrogen. The glass transition temperature of encapsulated shoot tips can be controlled by varying the hydration level. Deeper dehydration increases the glass transition temperature. There is a problem with the desiccation of plants, because at a certain level of dehydration the plant cells are irreversibly injured. In these experiments we used meristem shoot tips that were approximately 2 mm long, dissected from bulblets of garlic (Allium sativum L. acc. No.1669). The meristems were kept on agar with 0.6 M sucrose in petri dishes for 24 h. Thereafter, the meristems were encapsulated in 2% alginate containing 0.7 M sucrose. The alginate beads with the meristems were desiccated over saturated salt solutions or de (en)
  • The encapsulation–dehydration method is used for the cryopreservation of shoot tips or meristematic tissue of vegetative propagated plants. It is supposed that the shoot tips are able to survive due to the glassy state produced during rapid freezing of plant parts to the temperature of liquid nitrogen. The glass transition temperature of encapsulated shoot tips can be controlled by varying the hydration level. Deeper dehydration increases the glass transition temperature. There is a problem with the desiccation of plants, because at a certain level of dehydration the plant cells are irreversibly injured. In these experiments we used meristem shoot tips that were approximately 2 mm long, dissected from bulblets of garlic (Allium sativum L. acc. No.1669). The meristems were kept on agar with 0.6 M sucrose in petri dishes for 24 h. Thereafter, the meristems were encapsulated in 2% alginate containing 0.7 M sucrose. The alginate beads with the meristems were desiccated over saturated salt solutions or de (cs)
Title
  • Dehydratace a skelné přechody u enkapsulovaných vzrostných vrcholů česneku (cs)
  • Dehydration and glass transition of encapsulated garlic shoot tips
  • Dehydration and glass transition of encapsulated garlic shoot tips (en)
skos:prefLabel
  • Dehydratace a skelné přechody u enkapsulovaných vzrostných vrcholů česneku (cs)
  • Dehydration and glass transition of encapsulated garlic shoot tips
  • Dehydration and glass transition of encapsulated garlic shoot tips (en)
skos:notation
  • RIV/00027006:_____/01:1073!RIV06-GA0-00027006
http://linked.open.../vavai/riv/strany
  • 328;329
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/01/1399)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 677045
http://linked.open...ai/riv/idVysledku
  • RIV/00027006:_____/01:1073
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • dehydration, glass transition, encapsulation, garlic (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [B736F2734C7E]
http://linked.open...i/riv/nazevZdroje
  • Cryobiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 43
http://linked.open...iv/tvurceVysledku
  • Zámečník, Jiří
  • Bilavčík, Alois
  • Grospietsch, Martin
issn
  • 0011-2240
number of pages
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