About: Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes

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About: Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1002/jcb.23263
Description	The effects of the pan-caspase inhibitor Q-VD-OPh on caspase activity, DNA fragmentation, PARP cleavage, 7A6 exposition, and cellular adhesivity to fibronectin were analyzed in three different apoptotic systems involving two cell lines and two apoptosis inducers. Q-VD-OPh fully inhibited caspase-3 and -7 activity at 0.05 μM concentration, but significantly higher Q-VD-OPh dose (10 μM) was required to prevent the cleavage of PARP-1. DNA fragmentation and cell membrane permeabilization were both prevented at 2 μM Q-VD-OPh while 10 μM inhibitor was needed to inhibit the loss of cellular adhesivity to fibronectin. The exposition of the mitochondrial antigen 7A6 occurred independently of Q-VD-OPh addition and may serve to the detection of cumulative incidence of the cells which have initiated the apoptosis. Q-VD-OPh efficiency in the whole-cell environment is of two orders of magnitude higher than that of z-VAD-fmk and this is not due to a low permeability of the latter through the membrane The effects of the pan-caspase inhibitor Q-VD-OPh on caspase activity, DNA fragmentation, PARP cleavage, 7A6 exposition, and cellular adhesivity to fibronectin were analyzed in three different apoptotic systems involving two cell lines and two apoptosis inducers. Q-VD-OPh fully inhibited caspase-3 and -7 activity at 0.05 μM concentration, but significantly higher Q-VD-OPh dose (10 μM) was required to prevent the cleavage of PARP-1. DNA fragmentation and cell membrane permeabilization were both prevented at 2 μM Q-VD-OPh while 10 μM inhibitor was needed to inhibit the loss of cellular adhesivity to fibronectin. The exposition of the mitochondrial antigen 7A6 occurred independently of Q-VD-OPh addition and may serve to the detection of cumulative incidence of the cells which have initiated the apoptosis. Q-VD-OPh efficiency in the whole-cell environment is of two orders of magnitude higher than that of z-VAD-fmk and this is not due to a low permeability of the latter through the membrane (en)
Title	Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes (en)
skos:prefLabel	Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes (en)
skos:notation	RIV/00023736:_____/11:00009312!RIV12-MZ0-00023736
http://linked.open...avai/predkladatel	Ústav hematologie a krevní transfúze
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/09/1026), Z(MZ0UHKT2005)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Grebeňová, Dana Kuželová, Kateřina Brodská, Barbora
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav hematologie a krevní transfúze
http://linked.open...dnocenehoVysledku	195368
http://linked.open...ai/riv/idVysledku	RIV/00023736:_____/11:00009312
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Q-VD-OPh; z-VAD-fmk; apoptosis; Imatinib; SAHA (en)
http://linked.open.../riv/klicoveSlovo	SAHA apoptosis Imatinib Q-VD-OPh z-VAD-fmk
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[AC06EC3A4360]
http://linked.open...i/riv/nazevZdroje	Journal of Cellular Biochemistry
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Proteomic analysis of suberoylanilide hydroxamic acid (SAHA) effects on human leukemic cells
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	112
http://linked.open...iv/tvurceVysledku	Brodská, Barbora Grebeňová, Dana Kuželová, Kateřina
http://linked.open...ain/vavai/riv/wos	000296423000028
http://linked.open...n/vavai/riv/zamer	Význam molekulárně biologických vyšetření pro objasnění patogeneze a pro diagnostiku poruch krvetvorby a využití krvetvorné buňky v léčbě onemocnění krvetvorby a jiných tkání.
issn	0730-2312
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jcb.23263
is http://linked.open...avai/riv/vysledek of	Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes Dose-dependent effects of the caspase inhibitor Q-VD-OPh on different apoptosis-related processes

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