About: Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages

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About: Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the proinflammatory genes induced in macrophages during immune and immunopathological reactions are regulated by transcription factor NF kappa B. The aim of our study was to characterize changes in the expression of genes associated with NF kappa B activation and signalling in THP-1 human macrophages co-cultured with A549 respiratory epithelial cells. At least 4-fold upregulation of mRNA level was found in 29 of 84 tested genes including genes for multiple cytokines and chemokines, membrane antigens and receptors, and molecules associated with NF kappa B signalling. The mRNA induction was confirmed at the level of protein expression by evaluating the release of IL-6 and IL-8 and by ICAM-1 expression. Blocking of one NF kappa B subunit by p65 siRNA inhibited the production of IL-6 in both cell types while IL-8 release from THP-1 cells did not seem to be affected. Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the proinflammatory genes induced in macrophages during immune and immunopathological reactions are regulated by transcription factor NF kappa B. The aim of our study was to characterize changes in the expression of genes associated with NF kappa B activation and signalling in THP-1 human macrophages co-cultured with A549 respiratory epithelial cells. At least 4-fold upregulation of mRNA level was found in 29 of 84 tested genes including genes for multiple cytokines and chemokines, membrane antigens and receptors, and molecules associated with NF kappa B signalling. The mRNA induction was confirmed at the level of protein expression by evaluating the release of IL-6 and IL-8 and by ICAM-1 expression. Blocking of one NF kappa B subunit by p65 siRNA inhibited the production of IL-6 in both cell types while IL-8 release from THP-1 cells did not seem to be affected. (en)
Title	Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages (en)
skos:prefLabel	Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages (en)
skos:notation	RIV/00023001:_____/11:00002482!RIV12-MZ0-00023001
http://linked.open...avai/predkladatel	Institut klinické a experimentální mediciny
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ME 906), P(NS10524), Z(MZ0IKEM2005)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Jarešová, Marcela Brabcová, Eva Kolesár, Libor Stříž, Ilja Slavčev, Antonij Brabcová, Irena Sekerková, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Institut klinické a experimentální medicíny
http://linked.open...dnocenehoVysledku	197848
http://linked.open...ai/riv/idVysledku	RIV/00023001:_____/11:00002482
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Co-cultures; Cytokines; Epithelial cells; Macrophages; NF kappa B (en)
http://linked.open.../riv/klicoveSlovo	Co-cultures Epithelial cells NF kappa B Macrophages Cytokines
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[E0930D13854D]
http://linked.open...i/riv/nazevZdroje	Immunobiology
http://linked.open...in/vavai/riv/obor	EC
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20906 Monocyte subpopulations in kidney transplantation and regulatory mechanisms of human macrophage differentiation
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	216
http://linked.open...iv/tvurceVysledku	Brabcová, Irena Sekerková, Alena Brabcová, Eva Jarešová, Marcela Kolesár, Libor Liu, X D. Poole, J. A. Rennard, S. I. Slavčev, Antonij Stříž, Ilja
http://linked.open...ain/vavai/riv/wos	000296365700006
http://linked.open...n/vavai/riv/zamer	Výzkum kardiovaskulárních nemocí, diabetu a transplantace životně důležitých orgánů
issn	0171-2985
number of pages	7 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.imbio.2011.04.009
is http://linked.open...avai/riv/vysledek of	Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages Epithelial cells modulate genes associated with NF kappa B activation in co-cultured human macrophages

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