About: Renal activity of Akt kinase in obese Zucker rats

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Renalní aktivita Akt kinázy, exprese a fosforylace mTOR , aktivita eNO syntázy byly studovány u obézních potkanů kmene Zucker a štíhlých kontrol. Aktivita AKT byla zvýšena u obézních potkanů paralelně s hyperizulinémií. Ve svalech rozdíly v AKT nebyly zjištěny. U obézních potkanů byla zvýšena fosforylace mTOR. Akutní inhibice PI3K wortmaninem snížila renální aktivitu Akt a mTOR u obézních ale nikoliv u štíhlých potkanů. (cs) Renal cortical Akt was determined in obese Zucker rats (ZO), a model of DM2, and lean controls (ZL). We also studied expression and phosphorylation of the mammalian target of rapamycin (mTOR) and endothelial NO synthase (eNOS), molecules downstream of Akt in the insulin signaling cascade, and documented modulators of renal injury. Akt activity was measured by a kinase assay with GSK-3 as a substrate. Expression of phosphorylated (active) and total proteins was measured by immunoblotting and immunohistochemistry. Renal Akt activity was increased in ZO as compared to ZL rats, in parallel with progressive hyperinsulinemia. No differences in Akt were observed in the skeletal muscle. Corresponding to increases in Akt activity, ZO rats demonstrated enhanced phosphorylation of renal mTOR. Acute PI3K inhibition with wortmannin (100 mug/kg) attenuated renal Akt and mTOR activities in ZO, but not in ZL rats. Renal cortical Akt was determined in obese Zucker rats (ZO), a model of DM2, and lean controls (ZL). We also studied expression and phosphorylation of the mammalian target of rapamycin (mTOR) and endothelial NO synthase (eNOS), molecules downstream of Akt in the insulin signaling cascade, and documented modulators of renal injury. Akt activity was measured by a kinase assay with GSK-3 as a substrate. Expression of phosphorylated (active) and total proteins was measured by immunoblotting and immunohistochemistry. Renal Akt activity was increased in ZO as compared to ZL rats, in parallel with progressive hyperinsulinemia. No differences in Akt were observed in the skeletal muscle. Corresponding to increases in Akt activity, ZO rats demonstrated enhanced phosphorylation of renal mTOR. Acute PI3K inhibition with wortmannin (100 mug/kg) attenuated renal Akt and mTOR activities in ZO, but not in ZL rats. (en)
Title	Renal activity of Akt kinase in obese Zucker rats Renal activity of Akt kinase in obese Zucker rats (en) Renální aktivita Akt kinázy u obézních potkanů Zucker. (cs)
skos:prefLabel	Renal activity of Akt kinase in obese Zucker rats Renal activity of Akt kinase in obese Zucker rats (en) Renální aktivita Akt kinázy u obézních potkanů Zucker. (cs)
skos:notation	RIV/00023001:_____/08:00001897!RIV09-MZ0-00023001
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MZ0IKEM2005)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Pelikánová, Terezie Komers, Radko Kazdová, Ludmila Ždychová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Institut klinické a experimentální medicíny
http://linked.open...dnocenehoVysledku	392315
http://linked.open...ai/riv/idVysledku	RIV/00023001:_____/08:00001897
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	akt kinase; Zucker rats; NO synthase; diabetes; insulin (en)
http://linked.open.../riv/klicoveSlovo	insulin diabetes NO synthase Zucker rats akt kinase
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[41B84087EE3D]
http://linked.open...i/riv/nazevZdroje	Experimental biology and medicine (Maywood)
http://linked.open...in/vavai/riv/obor	FB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	233
http://linked.open...iv/tvurceVysledku	Komers, Radko Pelikánová, Terezie Kazdová, Ludmila Ždychová, Jana Anderson, S. Linsley, JN
http://linked.open...ain/vavai/riv/wos	000259729500005
http://linked.open...n/vavai/riv/zamer	Výzkum kardiovaskulárních nemocí, diabetu a transplantace životně důležitých orgánů
issn	1535-3702
number of pages	11 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Renal activity of Akt kinase in obese Zucker rats Renal activity of Akt kinase in obese Zucker rats Renal activity of Akt kinase in obese Zucker rats Renal activity of Akt kinase in obese Zucker rats

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