About: Cry 3A σ-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop

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About: Cry 3A σ-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://academicjournals.org/article/article1380799201_Lee%20et%20al.pd
Description	Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity. Bacillus thuringiensis Cry3A gene was redesigned for high expression in Norwegian spruce and the sequence was slightly modified to allow for simple N- and C- terminal deletions and domain II loop 1 exchange for synthetic oligos. Modified Cry3A toxins from 13 variants of the synthetic gene were expressed in Escherichia coli BL21 and their toxicity on spruce bark beetle larvae was tested using spruce bark sandwiches. Mutant toxins with N-terminal deletion and loop 1 duplication showed increased toxicity. (en)
Title	Cry 3A σ-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Cry 3A σ-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop (en)
skos:prefLabel	Cry 3A σ-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop Cry 3A σ-endotoxin gene mutagenized for enhanced toxicity to spruce bark beetle in a receptor binding loop (en)
skos:notation	RIV/00020702:_____/12:#0000698!RIV14-MZE-00020702
http://linked.open...avai/predkladatel	Výzkumný ústav lesního hospodářství a myslivosti, v.v.i.
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH71290)
http://linked.open...iv/cisloPeriodika	86
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Malá, Jana Modlinger, Roman Knížek, Miloš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav lesního hospodářství a myslivosti, v.v.i.
http://linked.open...dnocenehoVysledku	129107
http://linked.open...ai/riv/idVysledku	RIV/00020702:_____/12:#0000698
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bacillus thuringiensis; Ips typographus; Picea abies (en)
http://linked.open.../riv/klicoveSlovo	Bacillus thuringiensis Ips typographus Picea abies
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[85EA4A7FB6E0]
http://linked.open...i/riv/nazevZdroje	African Journal of Biotechnology
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of transgenic tissue lines of spruce (Picea abies) showing high toxicity towards bark beatle (Scolytidae) species.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Malá, Jana Modlinger, Roman Pavingerová, Daniela Vlasák, Josef Knížek, Miloš Bříza, Jindřich
issn	1684-5315
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.5897/AJB11.3806

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