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  • The envelope transmembrane glycoprotein gp41 and the core protein p25, of the ovine lentivirus Maedi - Visna virus are the main targets for a specific antibody response in vivo. The genes coding those two viral components were cloned individually into expression vector pET15b. The recombinant proteins (MVgp41 and MVp25) were efficiently expressed, purified as soluble protein (MVp25) or under denaturing conditions (MVgp41) and used as antigens in western blotting. Testing the antigens against 98 sheep serum samples and comparing the results with a commercial ID test made a preliminary assessment of the diagnostic potential. Our results confirm that transmembrane protein is an important immunological marker in Maedi-Visna infection and its use as antigen may enhance the validity of serological diagnosis of ovine lentiviruses. (en)
  • Transmembrßnovř glykoprotein gp41 a korovř protein p25 lentiviru ovcÝ Maedi-Visna jsou d¨le×itřmi strukturami viru, proti kterřm je namÝ°ena specifickß protilßtkovß odpový´ infikovanÚho hostitele. Geny kˇdujÝcÝ tyto dvý virovÚ struktury byly klonovßny do plazmidovÚho vektoru pET15b. RekombinantnÝ proteiny (MVgp41 a MVp25) byly exprimovßny v bakterißlnÝch bu˛kßch E. coli a purifikovßny na afinitnÝ koloný jako solubilnÝ protein(MVp25) a v denaturujÝcÝch podmÝnkßch (MVgp41). Oba proteiny byly pou×ity jako antigeny v imunoblotu a byly testovßny na souboru 98 ovŔÝch sÚr. ZÝskanÚ vřsledky byly porovnßny s vřsledky dosa×enřmi komerŔný dostupnřm imunodifuznÝm testem. Dosa×enÚ vřsledky prokßzaly, ×e transmembrßnovř glykoprotein je d¨le×itřm imunologickřm markerem infekce virem Maedi-Visna a jeho pou×itÝ m¨×e vřrazný zp°esnit diagnostiku onemocnýnÝ.
Title
  • Presentation of results in the 11. International Congress of Virology (en)
  • Prezentace výsledků na 11. mezinárodním virologickém kongresu
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  • PG99163
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  • Maedi-Visna, diagnostic, serology, recombinant antigen (en)
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  • Zaslßna pr¨bý×nß zprßva o °eÜenÝ projektu v roce 1998vŔetný podrobnÚho zd¨vodnýnÝ Ŕerpanřch finanŔnÝch prost°edk¨ na °eÜenÝ projektu v roce 1998 (cs)
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  • diagnostic
  • serology
  • Maedi-Visna
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