New information on plant reaction under stress condition, increasing knowledge on regulative network resulting in higher or lower sensitivity to changing environmental conditions may lead to the development of cultivated species genotypes that will provide stable anf high quality parameters of plant production. Currently several mechanisms that underly such phenotypes, namely in model plant Arabidopsis thaliana. Other plant e.g. monocotyledonous species are not so extensively characrised, reaction of different genotypes locally adopted are not exactly described. Such knowledge is important namely for breeding of economically important species. Characterisation of genes function underlying enzymes of saccharide metabolism under stres condition and their possible epigenetic changes (namely methylations) in leaves and crowns is the main aim of the project. It would be possible to compare regulations and linked to signalling pathways in different barley genotypes previously characterised in field a (en)
Charakterizace expresních profilů genů metabolismu sacharidů a jejich možných epigenetických změn za podmínek stresu, porovnání reakce kontrastních genotypů a výběr potenciálních markerů.
Projekt byl zaměřen na studium adaptace ječmene na chlad a mráz. Byla studována exprese genů souvisejících hladiny s metabolickým přizpůsobením listů a odnožovacích uzlů. Byly identifikovány rozdíly v expresi genů ovlivňujících hladinu cukrů mezi orgány a odrůdami. Byla zavedena metoda přesné kvalitikace těchto změn pomocí RTqPCR. Byly zavedeny metody detekce metylačních změn. (cs)
Project aimeed to study adaptation of barley to cold and chilling. Gene expression of genes related to metabolic adjustement of leaves and crowns was investigated. Differences in gene expression related to sugar content between the two organs were detected. Precise procedure for transctript quantification was introduced. DNA methylation studies were launched. (en)