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  • Cryopreservation is a powerful method for saving of plant germplasm biodiversity. Explant preculture, pretreatment of cryopreserved material and freezing method used determine efficacy of the cryopreservation procedure. Physiological state of explants, water content in their tissue, presence of cryoprotectants (plant vitrification solutions) and rate of sample cooling or warming influence plant regeneration after cryopreservation. Sample behavior during freezing is determined by forementioned factors. Differential scanning calorimetry (DSC) is a method of thermal analysis for measurement of thermal characteristics of the sample during cooling or warming. All cryopreservation methods have to avoid either intracellular crystallization or any crystallization in the sample. On the contrary, a detection of a glassy state in the material promises successful cryopreservation. DSC can measure thermal characteristic of the material prepared for cryopreservation including crystallization or melting and glass (en)
  • Cílem tohoto projektu je zlepšit teoretické znalosti o kryoprezervaci rostlin studiem biofyzikálních a fyziologických změn spojených s jejich odolností ke kryoprezervaci.
Title
  • Thermal analysis - a tool for cryopreservation efficiency improvement (en)
  • Termická analýza – nástroj pro zvýšení efektivity kryoprezervace
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  • OC08062
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  • cryopreservation; biodiversity; thermal analysis (en)
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  • Byly stanoveny termické vlastnosti u několika druhů plodin při různé hydrataci pletiv. Byl prokázán pozitivní vliv předkultivace explantátů se sacharosou a působení ředěných kryoprotektivních roztoků. (cs)
  • Thermal characteristics were determined in several crops in different levels of hydration. Positive effect of slow explants dehydration during preculture with sucrose and pretreatment with diluted vitrification solution was demonstrated. (en)
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  • cryopreservation
  • biodiversity
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