About: The molecular genetic analysis of the candidate genes responsible for cytoplasmic male sterility in Silene vulgaris     Goto   Sponge   NotDistinct   Permalink

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  • Recombination in plant mitochondrial genomes generates chimeric genes. Some products of these genes interfere with mitochondrial (mt) function. As the demand for respiration increases in the course of pollen development, mt misfunction prevents the production of viable pollen. Because chimeric genes responsible for pollen sterility reside in mt genome, they are termed cytoplasmic male sterility genes (CMS). Individuals with CMS genes could be females or hermaphrodites, depending on the presence of nuclear genes, which may restore male fertility. This cytonuclear determination of gender is a basis for plant reproduction system termed gynodioecy, which is wide-spread among angiosperms.CMS genes are well-investigated in crop plants, but mostly unknown in wild species. Silene vulgaris became a model for the study of gynodioecy in natural populations. Genetic evidence suggests that several various CMS genes exist in this species, but none has been identified yet. The candidate chimeric genes were revealed (en)
  • Ověření funkce chimerických mitochondriálních genů, které by mohly být zodpovědny za cytoplazmatickou pylovou sterilitu u silenky obecné.
Title
  • The molecular genetic analysis of the candidate genes responsible for cytoplasmic male sterility in Silene vulgaris (en)
  • Analýza genů, které určují cytoplazmatickou pylovou sterilitu u silenky obecné
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  • ME09035
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  • plant mitochondrial DNA (en)
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  • Silenka obecná je modelovým druhem pro výzkum rozmnožování rostlin v přírodních populacích. Geny zodpovědné za pylovou sterilitu jsou uloženy v mitochondriálním genomu. Využili jsme genomický přístup “sekvenování další generace” (NGS) k rozluštění úplné sekvence čtyř mitochondriálních genomů u rodu Silene. Nalezli jsme čtyři nové CMS kandidátské geny a studovali jejich expresi. Naše výsledky ukazu (cs)
  • Silene vulgaris became a model species for the investigation of reproduction system in natural populations. The genes responsible for male sterility are located in mitochondrial genomes. We adopted a genomic approach based on next generation sequencing (NGS) to read the complete genetic information of 4 mitochondrial genomes in Silene. We identified 4 new CMS candidate genes. Our results suggest (en)
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