About: Investigation of posttranslational modifications of proteins with carbonyl compounds by using MALDI-TOF mass spectrometry and capillary electrophoresis.     Goto   Sponge   NotDistinct   Permalink

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Description
  • Investigation of posttranslational modifications of proteins with carbonyl compounds by using MALDI-TOF mass spectrometry and capillary electrophoresis. In this project three types of protein modification will be studied: reactions of aldehydes with peptides and proteins, nonenzymatic glycations and enzymatic glycosylations. Capillary separation methods (capillary electrophoresis, isoelectric focusing, microcolumn liquid chromatography) will be used and further developed for this research in the combination with the techniques for characterization of modified compounds. special attention will be paid to Matrix-Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF). This technique will be an experimental common basis of the project. (en)
  • Hlavní cíle tohoto projektu jsou: 1) stanovení produktu a mechanismu reakcí mezi karbonylovými sloučeninami a aminoskupinami peptidů a bílkovin, 2) rozvoj účinných deglykosylačních metod pro charakterizaci sacharidu a glykokonjugátu., 3) charakterizace sacharidových částí glykoproteinu a peptidoglykanu.
Title
  • Investigation of posttranslational modifications of proteins with carbonyl compounds by using MALDI-TOF mass spectrometry and capillary electrophoresis. (en)
  • Studium posttranslačních modifikací bílkovin kabonylovými sloučeninami pomocí hmotnostní spektrometrie MALDI-TOF a kapilarní elektroforézy.
skos:notation
  • ME 230
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  • Capillary separation methods, mass spectrometry, MALDI, characterization of carbonyl modified compou (en)
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  • Idnetifikace ječmenných bílkovin a stanovení distribuce molekulových hmotností oligosacharidů. (cs)
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  • MALDI
  • mass spectrometry
  • Capillary separation methods
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