About: Molecular Mechanism of Antiproliferative Effects of Growth Regulators Cytokinins and Related Compounds

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Molecular Mechanism of Antiproliferative Effects of Growth Regulators Cytokinins and Related Compounds Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=GV204/96/K235
Description	Recently discovered unusual specifity of cytokinin derivative olomoucine OC; 2-(2-hydroxy-ethylamino)-6-benzylamino--9-methylpurin) toward cell cycle kinases (cdc2, cdk2, cdk5, ERK1) suggested that analogues of plant hormones can be used to inhibit certain steps of the cell cycle. Indeed herein, we have already showed that both %22M-phase Promoting Factor%22 and DNA synthesis are inhibited in vitro by olomoucine. Preliminary data drom plant protoplasts, sea urchin embryos, various mammlian cell lines iculture indicated, that OC arrests cell both at the G1/S and the G2/M boundaries; these effects are reversible. Therefore, it is reasonable to assume, that olomoucine can be considered a standard compound for the design of a new generation of anticancer compounds. The OC design and inhibitory activity will be futher improved by modifications on C2, C6 and N9. We sould like: (1) to summarise basic structure/activity relationships of OC/cdks interactions; (2) to develop suicide-type inhibitors, and (3) to (en) Nedávno objevená neobvyklá specifita a afinita cytokininového derivátu olomoucinu (OC,2-(2-hydroxy-ethylamino)-6-benzylamino--9-methylpurin) k cyklin-dpendentní kinázám (cdc2, cdk2, cdk5, ERK1) ukázala na možnost inhibice důležitých fází buněčného cyklu (přechody G1/S a G2/M). Následně jsme potvrdili, že olomoucin inhibuje %22M-phase promoting factor%22 a syntézu DNA in vitro. Předběžné výsledky u rostlinných protoplastů, embryí mořských ježků, řady savčích buněčných linií kultivovaných in vitro, ale i rozdílných nádorových linií ukázaly, že OC zablokuje buňky v G1S a G2M přechodech; tyto vlivy jsou reversibilní. Objev olomoucinu jako prvního účinného inhibitoru cdk logicky přináší vývoj nové generace protinádorových látek na antimitotickém základě. Naším cílem je: 1) objasnit základní vztahy mezi strukturou derivátů OC a jejich aktivitou v cdk testech, 2) zvýšit inhibiční aktivitu OC modifikacemi na C2, C6 a N9, 3) připravit ireverzibilní inhibitory cdk na bázi OC, 4) vyvinout novou generaci inhibi
Title	Molecular Mechanism of Antiproliferative Effects of Growth Regulators Cytokinins and Related Compounds (en) Studium molekulárních mechanismů antiproliferačních účinků derivátů růstových regulátorů - cytokininů
skos:notation	GV204/96/K235
http://linked.open...avai/cep/aktivita	Comprehensive projects
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/GV204%2F96%2FK235/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/GV204%2F96%2FK235/celkoveNaklady
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	600861
http://linked.open...ai/cep/hlavniObor	EA - Morfologické obory a cytologie
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open...ep/partnetrHlavni	Ústav experimentální botaniky AV ČR, v.v.i.
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	42 (xsd:int)
http://linked.open...enychVysledkuVRIV	42 (xsd:int)
http://linked.open...iciPoslednihoRoku	2001
http://linked.open...atUdajeProjZameru	2001
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open.../cep/vedlejsiObor	FR - Farmakologie a lékárnická chemie CE - Biochemie
http://linked.open...jektu+dodavatelem	Projekt byl zaměřen na výzkum inhibitorů cyklin-dependentních kináz (CDK), které představují novou generaci potenciálních cytostatik s vysokou specifitou. V rámci projektu byla vyvinuta a testována velká řada nových preparátů. Tyto látky byly podrobně zk (cs)
http://linked.open...tniCyklusProjektu	ZBBBKU
is http://linked.open...vavai/riv/projekt of	Molecular mechanism of cytokinin action in cell division cycle. Induction of apoptosis and regression of spontaneous dog melanoma following in vivo application of synthetic cyclin-dependent kinase inhibitor olomoucine. Inhibition of cyclin-dependent kinases by purine analoques. Crystal structure of human cdk2 complexed with roscovitine. Local specific expression of ipt gene and their control of cytokinin metabolism in tobacco plants (Nicotiana tabacum L.). Cytokinins as inhibitors of plant amine oxydase. Docking-based development of purine -like inhibitor of Cdk2. Control of tumor development in plants animals: A comparative treatise. Cytokinin oxidase: the key enzyme in the biodegradation of cytokinins. Control of tumour development in plants and animals: a comparative treatise. Control of tumour development in plants and animals: a comparative treatise. Cytokinin oxidase: the key enzyme in the biodegradation of cytokinins. Synthetic cyclin dependent kinase inhibitors. New generation of potent anti-cancer drugs. Molecular and biochemical properties of cytokinin dehydrogenase - enzyme activity degrading cytokinins in plants Rearrangement and Loss of Bromine Radical and CO from some Bromobenzyl Alcohols Folowing Electron Ionisation. Rearrangement and Loss of Bromine Radical and CO from some Bromobenzyl Alcohols Following Electron Ionisation. Protoemics approach in classifying the biochemical basis of the anticancer activity of the new olomoucine-derived synthetic cyclin-dependent kinase inhibitor, bohemine. Derivates of azapurine The structure of 6-(2-hydroxybenzylamino)purine acetic acid solvate. Study of endogenous cytokinin levels in synchronized Vicia faba L. root tips. Relationship of apoptotic rate to BCL-2 expression and proliferation in breast cancer. Relationship of apoptotic rate to BCL-2 expression and proliferation in bre Synthesis, characterization and biological activity of two nickes(II) complexes with 6-(2-chlorobenzylamino) purine. In vitro biotransformation of 2,6,9-trisubstituted purine-derived cyklin-dependent kinase inhibitor bohemine by mouse liver microsomes Treatment of Vicia faba root tip cells with specific inhibitors to cyclin-dependent kinases leads to abnormal spindle formation. Analogues of biologically active compounds VI* synthesis of some arylhydrargyriothio-compounds derived from purine and pyrazolo[3,4-d]pyrimidine. The influence of olomoucine and roscovitine on the production of matrix metalloproteinases and TIMP-1 by human diploid Fibroblasts: The correlation with the effect on proliferation. Molecular nature of cytokinin binding in ATP binding pocket of cyclin-dependent kinases. SCGE analysis of genomic damage induced in cultured tobacco suspension cells by three purine analogues - Olomoucine, Bohemine and Roscovitine and by hedrogen peroxide, and ethyl methanesulfonate. Docking-Based Development of Purine-like Inhibitors of Cdk2. Docking-Based Development of Purine-like Inhibitors of Cyclin-Dependent Kinase 2 Docking-Based Development of Purine-lide Inhibitors of Cyclin-Dependent Kinase-2. N6-alkyladenosines and adenines labelled with tritium. Aromatic cytokinins: Status and future prospects. Cytokinin-derived cyclin dependent kinase inhibitors. Synthesis and cdc2 activity of olomoucine and related compounds. Different degree of specificity of monoclonal antibodies against zeatin riboside. N/A The determination of adenosine by RIA assay in plants. Diagnostic algorithm for evalution of estrogen receptor functional ability in breast cancer. Meta-Topolin induces high shoot formation from leaf tobacco discs in vitro. Dynamic of endogenous cytokinins in synchronized Vicia faba L. root tip cells. The aromatic cytokinins. Conditional transgenic expression of the ipt gene indicates a function for cytokinins in paracrine signaling in whole tobacco plants. N/A
is http://linked.open...vavai/cep/projekt of	http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1996/ico%3A61389030 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1996/orjk%3A11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1996/orjk%3A15110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1997/ico%3A61388971 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1997/ico%3A61389030 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1997/orjk%3A11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1997/orjk%3A15110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1998/ico%3A61388971 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1998/ico%3A61389030 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1998/orjk%3A11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1998/orjk%3A15110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1999/ico%3A61388971 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1999/ico%3A61389030 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1999/orjk%3A11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1999/orjk%3A15110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/1999/orjk%3AG44 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/2000/ico%3A61388971 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/2000/ico%3A61389030 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/2000/orjk%3A11110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/2000/orjk%3A15110 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/2000/orjk%3AG44 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GV204%2F96%2FK235/2001/ico%3A61389030

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software