About: Synthesis of immunoactive glycosides by beta-N-acetylhexosaminidases

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=GP203/09/P024
Description	Hexosamines carrying a negatively charged moiety at C-6 are strong ligands of the activation receptors of natural killer cells. In this project, we aim to examine the impact of the nature of the charged moiety to the ligand efficiency. A range of disaccharides will be prepared from glycosides bearing various charged groups at C-6 in transglycosylation reactions, catalyzed by beta-N-acetylhexosaminidases. The enzymes with appropriate substrate specificity will be selected by screening of a glycosidase library. Their ability to accept the modified glycosides as substrates will be predicted by molecular modeling with beta-N-acetylhexosaminidase from Aspergillus oryzae. The products of transglycosylation reactions will be tested in competitive binding assays with the NKR-P1 and CD69 activation receptors. Thus, the structure-activity relationship between the nature of the charged group and the performance of the ligand will be identified. Furthermore, beta-N-acetylhexosaminidase from Talaromyces flavus, an enzyme of unique substrate specifity, will be purified and characterized. This data will form the basis for future sequencing and cloning of this enzyme. (en) 1. Syntéza modifikovaných substrátů beta-N-acetylhexosaminidas; 2. Screening na štěpení a transglykosylace, enzymová syntéza disacharidů; 3. Studie vazebné afinity k aktivačním receptorům NK buněk; 4. Purifikace a charakterizace hexosaminidasy z Talaromyces flavus; 5. Molekulární modelování
Title	Synthesis of immunoactive glycosides by beta-N-acetylhexosaminidases (en) Syntéza imunoaktivních glykosidů pomocí beta-N-acetylhexosaminidas
skos:notation	GP203/09/P024
http://linked.open...avai/cep/aktivita	Post-graduate (doctorate) grants
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/GP203%2F09%2FP024/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/GP203%2F09%2FP024/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2015-03-02 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	91732808
http://linked.open...ai/cep/hlavniObor	CE - Biochemie
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open...vai/cep/kategorie	ZV - Základní výzkum
http://linked.open.../cep/klicovaSlova	beta-N-acetylhexosaminidasa; enzymová syntéza; imunoaktivita; molekulární modelování (en)
http://linked.open...ep/partnetrHlavni	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	12 (xsd:int)
http://linked.open...enychVysledkuVRIV	12 (xsd:int)
http://linked.open...lneniVMinulemRoce	2011-04-16 (xsd:date)
http://linked.open.../prideleniPodpory	http://linked.opendata.cz/resource/domain/vavai/cep/prideleniPodpory/203%2F09%2FP024
http://linked.open...iciPoslednihoRoku	2011
http://linked.open...atUdajeProjZameru	2012
http://linked.open.../vavai/cep/soutez	SGA02009GA1PD
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open...ep/ukonceniReseni	2011-12-31 (xsd:date)
http://linked.open.../cep/vedlejsiObor	CC - Organická chemie EE - Mikrobiologie, virologie
http://linked.open...ep/zahajeniReseni	2009-01-01 (xsd:date)
http://linked.open...jektu+dodavatelem	The project was excellent, both in terms of expertise and in terms of disbursement of funds. (en) Řešení projektu proběhlo na vynikající úrovni, a to jak z hlediska odborného tak i z hlediska čerpání finančních prostředků. (cs)
http://linked.open...tniCyklusProjektu	ZBKU
http://linked.open.../cep/klicoveSlovo	enzymová syntéza imunoaktivita beta-N-acetylhexosaminidasa
is http://linked.open...vavai/riv/projekt of	Glycosidases: a key to tailored carbohydrates Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal β-N-acetylhexosaminidase Glycosidases in Carbohydrate Synthesis: When Organic Chemistry Falls Short Sequencing, cloning and high-yield expression of a fungal beta-N-acetylhexosaminidase in Pichia pastoris Synthesis of LacdiNAc-terminated glycoconjugates by mutant galactosyltransferase – A way to new glycodrugs and materials Glycosidases in synthesis Synthetic N-Acetyl-D-glucosamine Based Fully Branched Tetrasaccharide, a Mimetic of the Endogenous Ligand for CD69, Activates CD69+ Killer Lymphocytes upon Dimerization via a Hydrophilic Flexible Linker Beta-N-Acetylhexosaminidase: What's in a name? Cooperation between subunits is essential for high-affinity binding of N-acetyl-D-hexosamines to dimeric soluble and dimeric cellular forms of human CD69 4-Deoxy-substrates for B-N-acetylhexosaminidases: How to make use of their loose specificity Charged Hexosaminides as New Substrates for β-N-Acetylhexosaminidase-Catalyzed Synthesis of Immunomodulatory Disaccharides
is http://linked.open...vavai/cep/projekt of	http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GP203%2F09%2FP024/2009/ico%3A61388971 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GP203%2F09%2FP024/2010/ico%3A61388971 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GP203%2F09%2FP024/2011/ico%3A61388971 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GP203%2F09%2FP024/2012/ico%3A61388971

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