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  • During the maturation of Mason-Pfizer monkey virus polyprotein Gag precursor is cleaved by protease yielding six proteins: p10 (matrix protein - MA), pp24/18, p12, p27 (capsid protein - CA), p14 (nucleocapsid protein - NC) and p4. The functions of pp24/18, p12 and p4 proteins have not been sufficiently explained yet. The deletion mutation experiments in gene encoding protein p12 proved the hypothesis, that this protein supports the assembly of immature viral capsid. The aim of this project is to solve three-dimensional structure of N-terminal domain of protein p12, that could be utilized for a design of suitable inhibitor or viral capsid assembly. The protein will be expressed in E. coli BL21(DE3). Multidimensional NMR spectroscopy on isotopically labeled (15N or 15N,13C) samples together with computational methods will be employed for the solution of three-dimensional structure of this protein. (en)
  • Polyproteinově prekurzor Gag Mason-Pfizerova opiźˇho viru je bŘhem maturace rozçtŘpen proteasou na çest protein…: p10 (matrixově protein - MA), pp24/18, p12, p27 (kapsidově protein - CA), p14 (nukleokapsidově protein - NC) a p4. Funkce protein… pp24/18, p12 a p4 dosud nebyla uspokojivŘ vysvŘtlena. Metodou deleźnˇch mutagenezˇ v genu k˘dujˇcˇm protein p12 bylo prok z no, §e tento protein napom h  skl d nˇ nezral‚ virov‚ kapsidy. Cˇlem tohoto projektu je vyýeçit trojdimenzion lnˇ strukturu N-termin lnˇ dom‚ny proteinu p12, na jejˇm§ z kladŘ bude mo§n‚ navrhnout vhodně inhibitor skl d nˇ virov‚ kapsidy. Pro pýˇpravu proteinu bude pou§ita metoda exprese v E.coli BL21(DE3). K ýeçenˇ prostorov‚ struktury proteinu p12 bude vyu§ita vˇcedimenzion lnˇ NMR spektroskopie izotopovŘ obohaceněch (15N nebo 15N, 13C) vzork… spolu s věpoźetnˇmi metodami.
Title
  • Structural study of protein p12 from Mason-Pfizer monkey virus using NMR spectroscopy (en)
  • Strukturnˇ studie proteinu p12 Mason-Pfizerova opiźˇho viru pomocˇ NMR spektroskopie
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  • GP203/02/P098
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  • Odborně pýˇnos projektu lze spatýovat v objasnŘnˇ pýˇźin tvorby uspoý dan‚ struktury proteinu P12. édaje ýeçitele jsou zcela adekv tnˇ. Věznam projektu nem  pýˇmou aplikaci, pova§uji jej vçak za pýˇnos k pozn nˇ věznamu vyççˇch bˇlkovinněch struktur. Věs (cs)
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