About: Capillary electrophoresis as a member of the metabolomic analytical toolbox

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Projekt, within Data Space : linked.opendata.cz associated with source document(s)

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rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=GAP206/11/0009
Description	Bude vyvinuta nová "generická" metoda pro metabolomické studie založená na aplikaci různých módů CE pro odpovídající skupinu metabolitů. Ty budou kombinovány s relevantními on-line prekoncentračními technikami a detekcí pomocí MS. Metoda bude použitelná pro "metabolite fingerprinting" a "footprinting", "metabolite profiling" a pro "target metabolome analysis". K dispozici tak bude kompletní procedura pro mikrobiální metabolomické studie zahrnující rychlé zastavení metabolismu a kvantitativní extrakci vybraných metabolitů až po jejich analýzu různými módy CE. Kromě toho bude k dispozici metoda pro neinvazivní metabolomiku pro testování kvality embryí pro možnosti asistované reprodukce. The "generic" methods for metabolomic studies based on application of different CE modes – capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well. (en)
Title	Capillary electrophoresis as a member of the metabolomic analytical toolbox (en) Kapilární electroforéza jako nástroj pro metabolomické studie
skos:notation	GAP206/11/0009
http://linked.open...avai/cep/aktivita	Standard projects
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/GAP206%2F11%2F0009/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/GAP206%2F11%2F0009/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2015-04-23 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	100773207
http://linked.open...ai/cep/hlavniObor	CB - Analytická chemie, separace
http://linked.open...vai/cep/kategorie	ZV - Základní výzkum
http://linked.open.../cep/klicovaSlova	capillary electrophoresis MS NMR metabolomics systems biology biomarkers (en)
http://linked.open...ep/partnetrHlavni	Přírodovědecká fakulta
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	16 (xsd:int)
http://linked.open...enychVysledkuVRIV	16 (xsd:int)
http://linked.open...lneniVMinulemRoce	2014-03-31 (xsd:date)
http://linked.open.../prideleniPodpory	http://linked.opendata.cz/resource/domain/vavai/cep/prideleniPodpory/P206-11-0009
http://linked.open...iciPoslednihoRoku	2015
http://linked.open...atUdajeProjZameru	2015
http://linked.open.../vavai/cep/soutez	SGA02011GA-ST
http://linked.open...usZobrazovaneFaze	DRRVK
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open...ep/ukonceniReseni	2015-12-31 (xsd:date)
http://linked.open...ep/zahajeniReseni	2011-01-01 (xsd:date)
http://linked.open...tniCyklusProjektu	ZBBBK
is http://linked.open...vavai/riv/projekt of	Naphthalene-2,3-dicarbaldehyde derivatization of amino acids - an improved technique for minimization of benzoin condensation Simultaneous quantification of energetically important metabolites in various cell types by capillary zone electrophoresis Application of short-end injection procedure in capillary electrophoresis Amino acid profiling of human plasma samples using capillary electrophoresis with contactless conductivity detection Study of the most common additives affecting electro-osmotic flow for use in capillary electrophoresis with contactless conductivity detection setup Method development of amino acids profiling for pyridoxine dependent epileptic seizures diagnosis Metabolomics: nucleotide and coenzyme determination in cells by capillary electrophoresis Capillary electrophoresis with contactless conductivity detection for amino acid profiling of human plasma and urine samples Determination of asymmetric and symmetric dimethylarginines in human plasma using high-performance liquid chromatography with electrochemical detection RECEPTIVITA ENDOMETRIA V ASISTOVANÉ REPRODUKCI Capillary electrophoresis with contactless conductivity detection for amino acid profiling of human plasma samples Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection Metabolomika – základní pojmy, strategie a metodologie Utilization of the anodic electro-osmotic flow for an amino acids profiling by capillary electrophoresis Nonlinear Regression Models for Determination of Nicotinamide Adenine Dinucleotide Content in Human Embryonic Stem Cells The new technologies for the analytical examination of the embryonic metabolome and its prospects
is http://linked.open...vavai/cep/projekt of	http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GAP206%2F11%2F0009/2011/orjk%3A14310 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GAP206%2F11%2F0009/2012/orjk%3A14310 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GAP206%2F11%2F0009/2013/orjk%3A14310 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GAP206%2F11%2F0009/2014/orjk%3A14310 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/GAP206%2F11%2F0009/2015/orjk%3A14310

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