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| Description
| - The project is focused on the synthesis of N6-isopentenyladenine, cis-zeatin, trans-zeatin, N6-benzyladenine, 3-hydroxybenzyladenine and 4-hydroxybenzyladenine derivatives substituted in C2, C8 and N9 positions of the adenine moiety with the appropriate spacer-arm or suitable function group to design potentially biospecific ligands for affinity chromatography of cytokinin derivatives. The proposed project combines our knowledge of organic synthesis of cytokinine derivatives, the ability to study the biological activity of newly prepared derivatives in the extensive number of bioassays and very sensitive and modern quantitative proteomic techniques based on the combination of biochemical and analytical separation methods with highly sensitive mass spectrometry (MALDI-MS, nanoLC-ESI-MSMS) available at the place of work. Results of this project should lead to the identification of new binding and/or storage proteins that can play the crucial role in the regulation of germination and seedling development via modulation of cytokinin levels. (en)
- Projekt je zaměřen na syntézu derivátů N6-isopentenyladeninu, cis-zeatinu, trans-zeatinu, N6-benzyladeninu, 3-hydroxybenzyladeninu a 4-hydroxybenzyladeninu, substituovaných vhodným raménkem nebo vhodnou funkční skupinou konkrétně v polohách atomů C2, C8 a N9 adeninového skeletu za účelem designu účinných biospecifických ligandů vhodných pro afinitní chromatografii cytokininových derivátů. Navrhovaný projekt spojuje naše znalosti organické syntézy cytokininových derivátů, schopnost studia biologické aktivity nově připravených derivátů v rozsáhlé řadě biologických testů a zároveň dostupnost velmi citlivých a moderních technik kvantitativní proteomiky, založených na kombinaci biochemických a analytických separačních metod v kombinaci s vysoce citlivou hmotnostní spektrometrií (MALDI-MS, nano-LC-ESI-MSMS) na našem pracovišti. Výsledky z tohoto projektu povedou k identifikaci nových vazebných nebo zásobních proteinů, které mohou hrát klíčovou roli v regulaci klíčení a vývoje semenáčku cestou modulace hladin cytokininů.
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| Title
| - Development of biospecific ligands for affinity chromatography of cytokinin related proteins. (en)
- Vývoj biospecifických ligandů pro afinitní chromatografii proteinů schopných vázat cytokininy.
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - cytokinins; binding; proteins; organic; synthesis; affinity; chromatography; proteomics; mass; spectrometry (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...lneniVMinulemRoce
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| http://linked.open.../prideleniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open...ep/ukonceniReseni
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...ep/zahajeniReseni
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| http://linked.open...jektu+dodavatelem
| - - Byla připravena řada biologicky aktivních derivátů cytokininů pro bioafinitní chromatografii s cílem nalézt interakční partnery cytokininu. Přínosem je vytvořené metodické zázemí pro další výzkum v této oblasti. - Charakteristika je adekvátní - Deriváty jsou využívány v dalších oblastech výzkumua mají i aplikační potenciál - 4 publikace a 4 zahraniční patenty. - Nedostatky nebyly shledány (cs)
- -A number of bioactive cytokinine derivatives was synthetized for bioaffinity chromatography with the aim to reveal interacting proteins. Benefit is established methodical background for further studies. -Characteristic is adequate. -derivatives are used for research purposes and have application potential -4 original papers and 4 international patents -No fails found during the project course (en)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - cytokinins
- affinity
- binding
- chromatography
- mass
- organic
- proteins
- proteomics
- synthesis
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| is http://linked.open...vavai/riv/projekt
of | - Synthesis, characterization and biological activity of ring-substituted 6-benzylamino-9-tetrahydropyran-2-yl and 9-tetrahydrofuran-2-ylpurine derivatives
- X-ray structure, NMR and stability-in-solution study of 6-(furfurylamino)-9-(tetrahydropyran-2-yl)purine - A new active compound for cosmetology
- A new approach for cytokinin isolation from Arabidopsis tissues using miniaturized purification: pipette tip solid-phase extraction
- Pyrazolo[4,3-D]pyrimidines, processes for their preparation and methods for therapy
- Pyrazolo[4,3-D]pyrimidines, processes for their preparation and methods for therapy
- Nouveaux pyrazolo[4,3-D]pyrimidines, procedes pour leur preparation et methodes
- Heterocyclic compound based on N6-substituted adenine, methods of their preparation, their use for preparation of drugs, cosmetic preparations and growth regulators, pharmaceutical preparations, cosmetic preparations and growth regulators containing these compounds
- Heterocyclic compounds based on N6-substituted adenine, methods of their preparation, their use for preparation of drugs, cosmetic preparations and growth regulators pharmaceutical preparations, cosmetic preparations and growth regulators containing these compounds
- Seed development, seed germination and seedling growth in the R50 (sym16) pea mutant are not directly linked to altered cytokinin homeostasis
- Pyrazolo[4,3-D]pyrimidines, process for their preparation and methods of use
- Novel pyrazolo[4,3-D]pyrimidines, processes for their preparation and methods for therapy
- N9-substituted derivatives of kinetin: Effective anti-senescence agents
- N9-Substituted N(6)-[(3-methylbut-2-en-1-yl)amino]purine derivatives and their biological activity in selected cytokinin bioassays
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| is http://linked.open...vavai/cep/projekt
of | |
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