Stomata are crucial for productivity and survival of terrestrial plants. Leaf-atmosphere gas exchange is controlled by variable stomatal aperture, stomatal density and spatial distribution. Both density and the distribution are affected by frequency and regulation of asymmetric protedermal cell division; by cell-to-cell communication. In the last decade, important mechanisms leading to the spatial separation of stomata, the minimum one-celled spacing rule, were discovered. The mechanism is under both genetic and environmental control. Mutations in three main genes, TMM, FLP and SDD1 produced stomatal clusters and/or changes in density. Similar phenotypes are known also from in-vitro grown brasicaceae plants. We observed stomatal clusters in Lepidium plants grown in atmosphere with increased diffusivity (in helium and oxygen mixture), at high humidity and low CO2 concentrations. The main aim of the proposed project is to specify which environmental factors and physiological processes affect the signalling pathway leading to proper spatial arrangement of stomata in epidermis. (en)
Specifikovat parametry vnější atmosféry narušující prostorově správnou morgogenezi průduchů a zjistit zda jde o druhově specifický jev. Ověřit zda a jak produkce průduchových klastrů souvisí s respirací, propustností kutikuly, koncentrací CO2 v epidermis a expresí signálních proteinů.